在爱泼斯坦-巴尔病毒转化的B细胞系中生长的HIV-1变体对齐多夫定的敏感性。

Susceptibility to AZT of HIV-1 variants grown in Epstein-Barr virus-transformed B cell lines.

作者信息

Tremblay M, Wainberg M A

机构信息

Lady Davis Institute for Medical Research, Sir Mortimer B. Davis--Jewish General Hospital, Montreal, Quebec, Canada.

出版信息

J Infect Dis. 1989 Jul;160(1):31-6. doi: 10.1093/infdis/160.1.31.

DOI:10.1093/infdis/160.1.31

PMID:2543710

Abstract

How 3'-azido-3'-deoxythymidine (AZT) affects the ability of three different variants of HIV-1 to infect a line of EBV-transformed B cells was studied. Susceptible cells were pretreated with three different concentrations of AZT (1, 5, and 10 microM) for 4 h before viral inoculation and were maintained after infection in drug-containing medium. Establishment of viral infection was assessed by indirect immunofluorescence for viral antigens and by antigen capture assay carried out on culture fluids. AZT completely blocked infection in one of the HIV-1 variants studied; in the other two, treatment of cells with AZT significantly delayed the appearance of progeny virus.

摘要

研究了3'-叠氮-3'-脱氧胸苷（AZT）如何影响三种不同变体的HIV-1感染EBV转化的B细胞系的能力。在病毒接种前，将敏感细胞用三种不同浓度的AZT（1、5和10微摩尔）预处理4小时，并在感染后维持在含药物的培养基中。通过病毒抗原的间接免疫荧光和对培养液进行的抗原捕获试验来评估病毒感染的建立情况。AZT完全阻断了所研究的一种HIV-1变体的感染；在另外两种变体中，用AZT处理细胞显著延迟了子代病毒的出现。