Laboratório de Doenças Infectocontagiosas dos Animais Domésticos, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CP 52171-900 Recife, Pernambuco, Brazil.
Laboratório de Doenças Infectocontagiosas dos Animais Domésticos, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CP 52171-900 Recife, Pernambuco, Brazil.
Acta Trop. 2015 Feb;142:145-8. doi: 10.1016/j.actatropica.2014.11.011. Epub 2014 Nov 28.
The aim of the present study was to detect Toxoplasma gondii DNA in raw milk samples of goats and sheep of local breeds from the semi-arid region of the states of Pernambuco and Paraíba, Northeastern Brazil. Serum and milk samples were collected from 243 animals (186 goats and 57 sheep). The Indirect fluorescent antibody test (IFAT) was used to search for anti-T. gondii IgG antibodies with a cutoff of 64. Subsequently, the raw milk samples were subjected to DNA extraction and PCR to detect DNA of T. gondii. The IFAT results showed a 6.58% (16/243) positivity when all the samples were considered and a positivity of 15.78% (9/57) and 3.76% (7/186) for goats and sheep samples, respectively. The PCR assay detected T. gondii DNA in 2.06% (5/243) of all the samples tested. All the PCR positive samples were from goats. This result shows the importance of adopting measures of flock's sanitary management and avoiding the consumption of raw milk may constitute a potential risk to the health of milk consumers in this region.
本研究旨在检测巴西东北部伯南布哥州和帕拉伊巴州半干旱地区当地品种山羊和绵羊的生奶样本中的刚地弓形虫 DNA。从 243 只动物（186 只山羊和 57 只绵羊）中采集血清和奶样。采用间接荧光抗体试验（IFAT）检测抗刚地弓形虫 IgG 抗体，截断值为 64。随后，对生奶样本进行 DNA 提取和 PCR 检测刚地弓形虫 DNA。考虑到所有样本，IFAT 结果显示阳性率为 6.58%（16/243），山羊和绵羊样本的阳性率分别为 15.78%（9/57）和 3.76%（7/186）。PCR 检测显示，在检测的所有样本中，有 2.06%（5/243）检出刚地弓形虫 DNA。所有 PCR 阳性样本均来自山羊。该结果表明，采取羊群卫生管理措施并避免食用生奶对该地区奶消费者的健康构成潜在风险。
