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[Transposition of insertional sequence IS21 in E. coli cells activated by an exogenous promotor].

作者信息

Dubeĭkovskiĭ A N

出版信息

Mol Gen Mikrobiol Virusol. 1989 Jan(1):19-24.

PMID:2543920
Abstract

Transpositional activity of the IS21-element was found to be activated during its cloning in the pUC18 vector plasmid. The activated IS-element restores ability to transposition of the deficient IS21-element present in the same cell. Simultaneously with the activated transposition the increase in frequencies of precise exclusion of IS-element is registered. The activation of IS-element is concluded to be directed by exogenous promoter.

摘要

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