[利用噬菌体λ的oLpLN区域对IS1元件转座进行有效选择和分析]
[Effective selection and analysis of IS1 element transposition using the oLpLN region of phage lambda].
作者信息
Glukhov I L, Fodor I
出版信息
Mol Gen Mikrobiol Virusol. 1987 Sep(9):27-30.
PMID:2828939
Abstract
The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element insertion. The frequency of IS1 transposition into pL depends on genotype. The plasmid pNT6 may be considered to be a useful target DNA for screening and analysis of IS1-element transposition.
摘要
质粒pNT6允许选择以约10%的频率插入该质粒的IS1元件。克隆在pNT6中的噬菌体启动子pL是IS1元件插入的热点区域。IS1转座到pL中的频率取决于基因型。质粒pNT6可被认为是用于筛选和分析IS1元件转座的有用靶DNA。
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