Wagener K, Grunert T, Prunner I, Ehling-Schulz M, Drillich M
Clinical Unit for Herd Health Management in Ruminants, University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna 1210, Austria; Functional Microbiology, Institute of Bacteriology, Mycology and Hygiene, Department for Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna 1210, Austria.
Functional Microbiology, Institute of Bacteriology, Mycology and Hygiene, Department for Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, Vienna 1210, Austria.
Vet J. 2014 Dec;202(3):527-32. doi: 10.1016/j.tvjl.2014.08.023. Epub 2014 Aug 27.
The diversity and dynamics of the uterine microbiota of dairy cows are poorly understood although it is becoming increasingly evident that they play a crucial role in the development of metritis and endometritis. Fourier-transform infrared (FTIR) spectroscopy was used to monitor the bovine microbiota of 40 cows on the day of calving and days 3, 9, 15, and 21 after parturition, and to investigate the associations of selected species with clinical endometritis (CE). Trueperella pyogenes (43.5%), Escherichia coli (21.5%), Bacillus spp. (21.0%) and Streptococcus uberis (18.5%) were the most frequently isolated microbes. Analyses of different sampling time points revealed that the presence of S. uberis on day 3 increased the risk of subsequent T. pyogenes infection on day 9 (odds ratio [OR] = 5.1, 95% confidence interval [CI] = 1.2-22.6). T. pyogenes infection (OR = 36.0, 95% CI = 3.8-343.2) and retained fetal membranes (RFM) (OR = 12.4, 95%CI = 1.4-112.7) were significant risk factors for CE. Cows with S. uberis on day 3 tended to have greater odds of CE than S. uberis-negative cows (OR = 7.1, 95% CI = 0.9-55.6). Chemometric analysis revealed significant differences in the metabolic profile of S. uberis strains isolated from cows with different vaginal discharge scores. This is the first study showing the association of specific S. uberis subtypes with the uterine health status of post-partum dairy cows. The study demonstrates that uterine clearance is a highly dynamic process, during which time bacteria show distinct patterns of progression, and provides information about interactions between bacterial species involved in the occurrence of CE.
尽管越来越明显地认识到奶牛子宫微生物群在子宫炎和子宫内膜炎的发展中起着关键作用，但对其多样性和动态变化却知之甚少。采用傅里叶变换红外（FTIR）光谱法，在40头奶牛产犊当天以及产后第3、9、15和21天监测其微生物群，并研究特定菌种与临床子宫内膜炎（CE）之间的关联。化脓隐秘杆菌（43.5%）、大肠杆菌（21.5%）、芽孢杆菌属（21.0%）和乳房链球菌（18.5%）是最常分离出的微生物。对不同采样时间点的分析表明，第3天乳房链球菌的存在会增加随后第9天化脓隐秘杆菌感染的风险（优势比[OR]=5.1，95%置信区间[CI]=1.2-22.6）。化脓隐秘杆菌感染（OR=36.0，95%CI=3.8-343.2）和胎膜残留（RFM）（OR=12.4，95%CI=1.4-112.7）是CE的重要危险因素。第3天有乳房链球菌的奶牛患CE的几率往往高于无乳房链球菌的奶牛（OR=7.1，95%CI=0.9-55.6）。化学计量分析显示，从具有不同阴道分泌物评分的奶牛中分离出的乳房链球菌菌株的代谢谱存在显著差异。这是第一项显示特定乳房链球菌亚型与产后奶牛子宫健康状况之间关联的研究。该研究表明子宫清除是一个高度动态的过程，在此期间细菌呈现出不同的进展模式，并提供了有关参与CE发生的细菌物种之间相互作用的信息。
