• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

经典猪瘟病毒在猪脐静脉内皮细胞中诱导氧化应激。

Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells.

作者信息

He Lei, Zhang Yanming, Fang Yanqin, Liang Wulong, Lin Jihui, Cheng Min

机构信息

College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi, 712100, P.R. China.

Animal Disease and Public Security Academician Workstation of Henan province, The Key Lab of Animal Disease and Public security, Henan University of Science and Technology, Luoyang, 471003, P.R. China.

出版信息

BMC Vet Res. 2014 Dec 2;10:279. doi: 10.1186/s12917-014-0279-3.

DOI:10.1186/s12917-014-0279-3
PMID:25439655
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4270048/
Abstract

BACKGROUND

Classical swine fever virus (CSFV) infection causes significant losses of pigs, which is characterized by hemorrhage, disseminated intravascular coagulation and leucopenia. The swine vascular endothelial cell is a primary target cell for CSFV. The aim of this study was to determine the role of CSFV infection in inducing oxidative stress (OS) in vascular endothelial cells.

RESULTS

We demonstrated that CSFV infection induced oxidative stress in swine umbilical vein endothelial cells (SUVECs), characterized by the induction of reactive oxygen species (ROS) production and the elevations of porcine antioxidant proteins thioredoxin (Trx), peroxiredoxin-6 (PRDX-6) and heme oxygenase-1 (HO-1) expression. Furthermore, cyclooxygenase-2 (COX-2), a pro-inflammatory protein related to oxidative stress, was up-regulated while anti-inflammatory protein peroxisome proliferator-activated receptor-γ (PPAR-γ), an important mediator in vascular functional regulation, was down-regulated in the CSFV infected cells. In addition, antioxidants showed significant inhibitory effects on the CSFV replication, indicating a close relationship between CSFV replication and OS induced in the host cells.

CONCLUSIONS

Our results indicated that CSFV infection induced oxidative stress in SUVECs. These findings provide novel information on the mechanism by which CSFV can alter intracellular events associated with the viral infection.

摘要

背景

经典猪瘟病毒（CSFV）感染会给猪造成重大损失，其特征为出血、弥散性血管内凝血和白细胞减少。猪血管内皮细胞是CSFV的主要靶细胞。本研究的目的是确定CSFV感染在诱导血管内皮细胞氧化应激（OS）中的作用。

结果

我们证明CSFV感染可诱导猪脐静脉内皮细胞（SUVECs）产生氧化应激，其特征为活性氧（ROS）生成增加以及猪抗氧化蛋白硫氧还蛋白（Trx）、过氧化物酶-6（PRDX-6）和血红素加氧酶-1（HO-1）表达升高。此外，与氧化应激相关的促炎蛋白环氧化酶-2（COX-2）在CSFV感染的细胞中上调，而血管功能调节中的重要介质抗炎蛋白过氧化物酶体增殖物激活受体-γ（PPAR-γ）下调。此外，抗氧化剂对CSFV复制具有显著抑制作用，表明CSFV复制与宿主细胞中诱导的OS之间存在密切关系。

结论

我们的结果表明CSFV感染可诱导SUVECs产生氧化应激。这些发现为CSFV改变与病毒感染相关的细胞内事件的机制提供了新信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/45dadc9d88e0/12917_2014_279_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/a42955b09046/12917_2014_279_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/9ba176dac793/12917_2014_279_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/184343b62bfa/12917_2014_279_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/b5fa6d3c9ab9/12917_2014_279_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/3b8bb8c8638b/12917_2014_279_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/be091f789c76/12917_2014_279_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/45dadc9d88e0/12917_2014_279_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/a42955b09046/12917_2014_279_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/9ba176dac793/12917_2014_279_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/184343b62bfa/12917_2014_279_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/b5fa6d3c9ab9/12917_2014_279_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/3b8bb8c8638b/12917_2014_279_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/be091f789c76/12917_2014_279_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c300/4270048/45dadc9d88e0/12917_2014_279_Fig7_HTML.jpg

相似文献

1
Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells.经典猪瘟病毒在猪脐静脉内皮细胞中诱导氧化应激。
BMC Vet Res. 2014 Dec 2;10:279. doi: 10.1186/s12917-014-0279-3.
2
Classical swine fever virus NS5A protein localizes to endoplasmic reticulum and induces oxidative stress in vascular endothelial cells.经典猪瘟病毒NS5A蛋白定位于内质网并在血管内皮细胞中诱导氧化应激。
Virus Genes. 2012 Oct;45(2):274-82. doi: 10.1007/s11262-012-0773-2. Epub 2012 Jun 21.
3
The effect of classical swine fever virus NS5A and NS5A mutants on oxidative stress and inflammatory response in swine testicular cells.经典猪瘟病毒NS5A及NS5A突变体对猪睾丸细胞氧化应激和炎症反应的影响
Res Vet Sci. 2017 Jun;112:89-96. doi: 10.1016/j.rvsc.2017.01.007. Epub 2017 Jan 13.
4
MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells.miR-140 通过靶向猪脐静脉内皮细胞中的 Rab25 抑制古典猪瘟病毒复制。
Virulence. 2020 Dec;11(1):260-269. doi: 10.1080/21505594.2020.1735051.
5
Classical swine fever virus NS2 protein promotes interleukin-8 expression and inhibits MG132-induced apoptosis.经典猪瘟病毒NS2蛋白促进白细胞介素-8表达并抑制MG132诱导的细胞凋亡。
Virus Genes. 2011 Jun;42(3):355-62. doi: 10.1007/s11262-011-0582-z. Epub 2011 Feb 12.
6
Proteomic analysis of primary porcine endothelial cells after infection by classical swine fever virus.经典猪瘟病毒感染后猪原代内皮细胞的蛋白质组学分析
Biochim Biophys Acta. 2010 Sep;1804(9):1882-8. doi: 10.1016/j.bbapap.2010.05.011. Epub 2010 Jun 1.
7
Classical Swine Fever Virus Infection and Its NS4A Protein Expression Induce IL-8 Production through MAVS Signaling Pathway in Swine Umbilical Vein Endothelial Cells.经典猪瘟病毒感染及其NS4A蛋白表达通过MAVS信号通路诱导猪脐静脉内皮细胞产生白细胞介素-8 。
Front Microbiol. 2018 Jan 12;8:2687. doi: 10.3389/fmicb.2017.02687. eCollection 2017.
8
Down-regulation of cellular protein heme oxygenase 1 inhibits proliferation of classical swine fever virus in PK-15 cells.细胞色素 P450 血红素加氧酶 1 的下调抑制 PK-15 细胞中经典猪瘟病毒的增殖。
Virus Res. 2013 May;173(2):315-20. doi: 10.1016/j.virusres.2013.01.012. Epub 2013 Jan 25.
9
Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus.硫氧还蛋白2是一种新型的与E2相互作用的蛋白，可抑制经典猪瘟病毒的复制。
J Virol. 2015 Aug;89(16):8510-24. doi: 10.1128/JVI.00429-15. Epub 2015 Jun 3.
10
Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro.猪 viperin 蛋白可抑制猪瘟病毒（classical swine fever virus，CSFV）在体外的复制。
Virol J. 2017 Oct 23;14(1):202. doi: 10.1186/s12985-017-0868-4.

引用本文的文献

1
Strategies of Classical Swine Fever Immune Evasion.经典猪瘟免疫逃逸策略
Int J Mol Sci. 2025 Aug 14;26(16):7838. doi: 10.3390/ijms26167838.
2
High Hcy regulates fluid shear stress pathway activity through histone H3K79 homocysteinylation in hyperhomocysteinemia-related child hypertension.高同型半胱氨酸通过组蛋白H3K79同型半胱氨酸化在高同型半胱氨酸血症相关儿童高血压中调节流体切应力通路活性。
J Transl Med. 2025 May 9;23(1):525. doi: 10.1186/s12967-025-06483-6.
3
Peroxiredoxins-The Underrated Actors during Virus-Induced Oxidative Stress.过氧化物酶-病毒诱导的氧化应激中被低估的参与者。

本文引用的文献

1
Low production of reactive oxygen species in granulocytes is associated with organ damage in systemic lupus erythematosus.粒细胞中活性氧生成不足与系统性红斑狼疮的器官损伤有关。
Arthritis Res Ther. 2014 Jun 5;16(3):R120. doi: 10.1186/ar4575.
2
RNA Viruses: ROS-Mediated Cell Death.RNA病毒：活性氧介导的细胞死亡
Int J Cell Biol. 2014;2014:467452. doi: 10.1155/2014/467452. Epub 2014 May 8.
3
Oxidative stress in angiogenesis and vascular disease.氧化应激与血管生成和血管疾病。
Antioxidants (Basel). 2021 Jun 18;10(6):977. doi: 10.3390/antiox10060977.
4
Apoptosis, Autophagy, and Pyroptosis: Immune Escape Strategies for Persistent Infection and Pathogenesis of Classical Swine Fever Virus.凋亡、自噬与焦亡：经典猪瘟病毒持续感染及发病机制的免疫逃逸策略
Pathogens. 2019 Nov 16;8(4):239. doi: 10.3390/pathogens8040239.
5
Classical Swine Fever Virus Infection and Its NS4A Protein Expression Induce IL-8 Production through MAVS Signaling Pathway in Swine Umbilical Vein Endothelial Cells.经典猪瘟病毒感染及其NS4A蛋白表达通过MAVS信号通路诱导猪脐静脉内皮细胞产生白细胞介素-8 。
Front Microbiol. 2018 Jan 12;8:2687. doi: 10.3389/fmicb.2017.02687. eCollection 2017.
6
Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV .黄芪多糖对猪瘟病毒刺激的内皮细胞黏附相关免疫反应的影响
PeerJ. 2017 Oct 6;5:e3862. doi: 10.7717/peerj.3862. eCollection 2017.
7
CSFV induced mitochondrial fission and mitophagy to inhibit apoptosis.猪瘟病毒诱导线粒体分裂和线粒体自噬以抑制细胞凋亡。
Oncotarget. 2017 Jun 13;8(24):39382-39400. doi: 10.18632/oncotarget.17030.
8
Inducible GBP5 Mediates the Antiviral Response via Interferon-Related Pathways during Influenza A Virus Infection.诱导型 GBP5 通过干扰素相关途径在甲型流感病毒感染中介导抗病毒反应。
J Innate Immun. 2017;9(4):419-435. doi: 10.1159/000460294. Epub 2017 Apr 5.
9
Multiple Functions of Glutamate Uptake via Meningococcal GltT-GltM L-Glutamate ABC Transporter in Neisseria meningitidis Internalization into Human Brain Microvascular Endothelial Cells.通过脑膜炎奈瑟菌GltT - GltM L - 谷氨酸ABC转运体摄取谷氨酸在脑膜炎奈瑟菌内化进入人脑微血管内皮细胞中的多种功能
Infect Immun. 2015 Sep;83(9):3555-67. doi: 10.1128/IAI.00654-15. Epub 2015 Jun 22.
10
Important Role of the IL-32 Inflammatory Network in the Host Response against Viral Infection.白细胞介素-32炎症网络在宿主抗病毒感染反应中的重要作用。
Viruses. 2015 Jun 16;7(6):3116-29. doi: 10.3390/v7062762.
Blood. 2014 Jan 30;123(5):625-31. doi: 10.1182/blood-2013-09-512749. Epub 2013 Dec 3.
4
Inflammation and oxidative stress in angiogenesis and vascular disease.炎症和氧化应激在血管生成和血管疾病中的作用。
J Mol Med (Berl). 2013 Mar;91(3):323-8. doi: 10.1007/s00109-013-1007-3. Epub 2013 Feb 22.
5
Thioredoxins, glutaredoxins, and peroxiredoxins--molecular mechanisms and health significance: from cofactors to antioxidants to redox signaling.硫氧还蛋白、谷氧还蛋白和过氧化物酶——分子机制和健康意义：从辅因子到抗氧化剂再到氧化还原信号。
Antioxid Redox Signal. 2013 Nov 1;19(13):1539-605. doi: 10.1089/ars.2012.4599. Epub 2013 Mar 28.
6
Classical swine fever virus NS5A protein localizes to endoplasmic reticulum and induces oxidative stress in vascular endothelial cells.经典猪瘟病毒NS5A蛋白定位于内质网并在血管内皮细胞中诱导氧化应激。
Virus Genes. 2012 Oct;45(2):274-82. doi: 10.1007/s11262-012-0773-2. Epub 2012 Jun 21.
7
Inflammation, oxidative stress, and obesity.炎症、氧化应激与肥胖。
Int J Mol Sci. 2011;12(5):3117-32. doi: 10.3390/ijms12053117. Epub 2011 May 13.
8
Attenuation of nitric oxide bioavailability in porcine aortic endothelial cells by classical swine fever virus.猪瘟病毒对猪主动脉内皮细胞一氧化氮生物利用度的抑制作用。
Arch Virol. 2011 Jul;156(7):1151-60. doi: 10.1007/s00705-011-0972-5. Epub 2011 Mar 22.
9
Anti-hepatitis C virus activity of Acacia confusa extract via suppressing cyclooxygenase-2.金合欢提取物通过抑制环氧化酶-2抗丙型肝炎病毒活性。
Antiviral Res. 2011 Jan;89(1):35-42. doi: 10.1016/j.antiviral.2010.11.003. Epub 2010 Nov 12.
10
Oxidative stress, inflammation, and cancer: how are they linked?氧化应激、炎症与癌症：它们之间有何关联？
Free Radic Biol Med. 2010 Dec 1;49(11):1603-16. doi: 10.1016/j.freeradbiomed.2010.09.006. Epub 2010 Sep 16.