猪圆环病毒样病毒P1的转录分析

Transcriptional analysis of porcine circovirus-like virus P1.

作者信息

Wen Li-bin, Wang Feng-zhi, He Kong-wang, Li Bin, Wang Xiao-min, Guo Rong-li, Xie Jian-ping

机构信息

Jiangsu Academy of Agricultural Sciences · Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture · National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Nanjing, 210014, China.

出版信息

BMC Vet Res. 2014 Dec 2;10:287. doi: 10.1186/s12917-014-0287-3.

DOI:10.1186/s12917-014-0287-3

PMID:25440084

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4258304/

Abstract

BACKGROUND

Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts.

RESULTS

The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses. Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids).

CONCLUSIONS

We show a very complex viral transcription pattern in P1-infected cells.

摘要

背景

最近发现的猪圆环病毒样病毒P1拥有最小的DNA病毒基因组。在本研究中，我们鉴定了该病毒的基因及其相应的mRNA转录本。

结果

对在猪肾细胞中合成的P1 RNA进行了Northern印迹和PCR分析。检测到8种病毒特异性RNA。4种mRNA（开放阅读框（ORF）1、2、4和5）由病毒（-）链编码，4种（ORF 3、6、7和8）由病毒（+）链编码。P1病毒ORF编码的所有蛋白质长度均小于50个氨基酸，但ORF1编码的蛋白质除外（113个氨基酸）。