Yoshimatsu T, Nagawa F
Institute for Biotechnology Research, Wakunaga Pharmaceutical Co., Ltd., Hiroshima, Japan.
Science. 1989 Jun 16;244(4910):1346-8. doi: 10.1126/science.2544026.
Artificial yeast introns that show cold-sensitive splicing have been constructed. These conditional introns can be inserted into a target gene as an "intron cassette" without disrupting the coding information, allowing expression of the gene to be cold sensitive. Insertion of these intron cassettes rendered the yeast URA3 gene cold sensitive in its expression. The advantage of this intron-mediated control system is that any gene can be converted to a controllable gene by simple insertion of an intron.
已构建出表现出冷敏感剪接的人工酵母内含子。这些条件性内含子可作为“内含子盒”插入目标基因，而不破坏编码信息，从而使该基因的表达对温度敏感。插入这些内含子盒使酵母URA3基因的表达对温度敏感。这种内含子介导的控制系统的优点是，通过简单插入一个内含子，任何基因都可以转化为一个可控基因。
