Sun Shuangjiao, Long Chanjuan, Tao Chunyao, Meng Sa, Deng Biyang
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Anal Chim Acta. 2014 Dec 3;851:37-42. doi: 10.1016/j.aca.2014.08.012. Epub 2014 Aug 13.
The paper describes a homemade ultrasonic microdialysis device coupled with capillary electrophoresis electrochemiluminescence (CE-ECL) for studying the interaction between human serum albumin (HSA) and trimetazidine dihydrochloride (TMZ). The time required for equilibrium by ultrasonic microdialysis was 45min, which was far less than that by traditional dialysis (240min). It took 80min to achieve the required combination equilibrium by normal incubation and only 20min by ultrasonic. Compared with traditional dialysis, the use of ultrasonic microdialysis simplified experimental procedures, shortened experimental time and saved consumption of sample. A simple, sensitive and selective determination of TMZ was developed using CE-ECL and the parameters that affected ECL intensity were optimized. Under the optimized conditions, the linear range of TMZ was from 0.075 to 80μmol/L (r(2)=0.9974). The detection limit was 26nmol/L with RSD of 2.8%. The number of binding sites and binding constant were 1.54 and 15.17L/mol, respectively.
该论文描述了一种自制的超声微透析装置与毛细管电泳电化学发光（CE-ECL）联用，用于研究人血清白蛋白（HSA）与盐酸曲美他嗪（TMZ）之间的相互作用。超声微透析达到平衡所需时间为45分钟，远少于传统透析所需的240分钟。正常孵育达到所需结合平衡需要80分钟，而超声作用下仅需20分钟。与传统透析相比，超声微透析的使用简化了实验步骤，缩短了实验时间并节省了样品消耗。利用CE-ECL建立了一种简单、灵敏且选择性的TMZ测定方法，并对影响ECL强度的参数进行了优化。在优化条件下，TMZ的线性范围为0.075至80μmol/L（r(2)=0.9974）。检测限为26nmol/L，相对标准偏差为2.8%。结合位点数和结合常数分别为1.54和15.17L/mol。
