印楝水提物通过增强活性氧发挥抗丝虫作用。
In vitro antifilarial activity of Azadirachta indica aqueous extract through reactive oxygen species enhancement.
机构信息
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, West Bengal, India.
出版信息
Asian Pac J Trop Med. 2014 Nov;7(11):841-8. doi: 10.1016/S1995-7645(14)60147-4. Epub 2014 Oct 22.
OBJECTIVE
To evaluate an aqueous preparation from the Azadirachta indica leaves (AEA) against Setaria cervi (S. cervi), a model filarial parasite.
METHOD
In vitro efficacy of AEA was evaluated against S. cervi through estimation of relative motility value, dye exclusion test and MTT assay. Visible morphological alterations were monitored using conventional microscopic techniques in microfilariae and haematoxylin-eosin stained sections of AEA-treated adults.
RESULTS
Enhancement of reactive oxygen species in S. cervi treated with AEA was established through alteration in the activity of glutathione S-transferase, superoxide dismutase, catalase, peroxidase and level of superoxide anion and reduced glutathione.
CONCLUSION
In vitro filaricidal activity of AEA is possibly through disturbing redox homeostasis by down-regulating and altering the level of some key antioxidants and regulatory enzymes like reduced glutathione, glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase of S. cervi.
目的
评估印楝叶的水提物（AEA）对丝状寄生虫马氏血吸虫（S. cervi）的作用。
方法
通过相对运动值评估、染料排除试验和 MTT 测定，评估 AEA 对 S. cervi 的体外疗效。在微丝蚴和用 AEA 处理的成虫的苏木精-伊红染色切片中，使用常规显微镜技术监测可见的形态学改变。
结果
通过改变谷胱甘肽 S-转移酶、超氧化物歧化酶、过氧化氢酶、过氧化物酶和超氧阴离子和还原型谷胱甘肽的活性，确定 AEA 处理的 S. cervi 中活性氧的增加。
结论
AEA 的体外杀丝蚴活性可能是通过下调和改变 S. cervi 中一些关键抗氧化剂和调节酶的水平，如还原型谷胱甘肽、谷胱甘肽 S-转移酶、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶，从而扰乱氧化还原稳态。
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