Kausar Sharba
Section of Parasitology, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh India.
J Parasit Dis. 2017 Mar;41(1):9-15. doi: 10.1007/s12639-015-0742-5. Epub 2016 Jan 13.
Polar solvent extracts of leaves were investigated to evaluate in vitro anthelmintic activity on the microfilariae of . Four extracts of leaves were prepared with different solvents in increasing order of polarity (diethyl ether < chloroform < ethanol < methanol). Stock solution of the extract of leaves was prepared in Ringer's solution at the concentrations of 50, 100 and 200 μg/ml and used against microfilariae in three replicates. Ringer's solution was used as experimental control. Mortality rates of microfilariae were recorded for each concentration after 45, 90 and 135 min, respectively. All the solvent extracts show significant anthelmintic activity at the concentration of 200 μg/ml after 135 min of incubation. Highest mortality rate of microfilariae was observed in methanol and ethanol extracts throughout the incubation period compared to chloroform and diethyl ether extracts which showed low mortality rates. Dose dependent effect of ethanol and chloroform showed highest and lowest ranking when analyzed by regression values and correlation of regression. Solvents extracts of leaves showed anthelmintic effect and therefore may be used as phytopharmacological agents in future.
对树叶的极性溶剂提取物进行了研究，以评估其对[某种寄生虫]微丝蚴的体外驱虫活性。用极性递增的不同溶剂（乙醚＜氯仿＜乙醇＜甲醇）制备了四种树叶提取物。树叶提取物的储备液在林格氏溶液中按50、100和200μg/ml的浓度制备，并用于对微丝蚴进行三次重复实验。林格氏溶液用作实验对照。分别在45、90和135分钟后记录每种浓度下微丝蚴的死亡率。孵育135分钟后，所有溶剂提取物在200μg/ml的浓度下均显示出显著的驱虫活性。在整个孵育期内，与死亡率较低的氯仿和乙醚提取物相比，甲醇和乙醇提取物中微丝蚴的死亡率最高。通过回归值和回归相关性分析，乙醇和氯仿的剂量依赖性效应分别显示出最高和最低的排名。树叶的溶剂提取物显示出驱虫作用，因此未来可能用作植物药理学药物。
