Müller W E, Wenger R, Reuter P, Renneisen K, Schröder H C
Abteilung Angewandte Molekularbiologie, Universität, Mainz, F.R.G.
Biochim Biophys Acta. 1989 Jul 7;1008(2):208-12. doi: 10.1016/0167-4781(80)90011-1.
The transactivating protein from human immunodeficiency virus type 1 (HIV-1), Tat, was found to bind to the nuclear matrix from uninfected and HIV-1-infected H9 cells. Addition of the Zn2+, Cd2+ and Cu2+ chelator o-phenanthroline destroyed the matrix fibrils and the binding affinity of Tat to the matrix. A sequential treatment of the matrix, first with o-phenanthroline and then with ZnCl2, partially restored the fibrillar-like matrix structure. Infection of H9 cells with HIV-1 resulted in a displacement of cellular mRNA by viral mRNA from the nuclear matrix. Both the matrix-bound host cell and HIV-1 mRNA were found to dissociate from the matrix in the presence of o-phenanthroline. This could be prevented by coincubation with Zn2+ or Cu2+ (but not Mg2+), which stabilize the mRNA containing nuclear matrix structure.
人们发现，来自1型人类免疫缺陷病毒（HIV-1）的反式激活蛋白Tat可与未感染及感染了HIV-1的H9细胞的核基质结合。添加锌离子、镉离子和铜离子螯合剂邻菲罗啉会破坏基质纤维以及Tat与基质的结合亲和力。先用邻菲罗啉处理基质，再用氯化锌处理，可部分恢复类似纤维状的基质结构。HIV-1感染H9细胞会导致病毒mRNA将细胞mRNA从核基质上置换下来。在邻菲罗啉存在的情况下，发现与基质结合的宿主细胞mRNA和HIV-1 mRNA都会从基质上解离。与锌离子或铜离子（而非镁离子）共同孵育可防止这种情况发生，因为锌离子或铜离子可稳定含有mRNA的核基质结构。
