Zhang T S
Zhonghua Liu Xing Bing Xue Za Zhi. 1989 Apr;10(2):74-7.
In order to provide serological evidence for arbovirus infection in Yunnan province, 760 Samples of human sera collected from nine counties in south-western part of Yunnan province were examined for HI antibodies to 11 arboviral antigens. The viruses used in this experiment included 3 alphaviruses (MAY, VEE and CHIK) and 8 flaviviruses (JE, MVE, KUN, DEN3, DEN4, KFD, LGT and POW). 275 samples were found positive for HI antibodies to alphaviruses (36.2%) and 189 of them (68.7%) reacted with MAY virus, 61 (22.2%) with CHIK. The GMT of HI titers for VEE, MAY and Chik were 164.4, 94.5 and 66.7, respectively. 588 samples of sera (77.4%) were found positive for HI antibodies to flaviviruses. The positive HI antibody rates were as follows: JE, 27.9%; DEN, 36.6%; KFD, 22.2%; MVE, 22.1%; KUN, 18.7%; POW, 8.8% and LGT, 7.9%, respectively. Its average GMT was 356, and the antibody titers of 403 samples of sera were higher than 1/640. Cross reactions among viruses, especially flaviviruses, were usually found by HI test, and superinfections were present. In addition to the existence of JE and DEN viruses the results clearly showed that many Kinds of arboviruses might exist in Yunan Province.
为了提供云南省虫媒病毒感染的血清学证据，对从云南省西南部9个县采集的760份人血清样本进行检测，以检测其对11种虫媒病毒抗原的血凝抑制（HI）抗体。本实验所用病毒包括3种甲病毒（马亚病毒、委内瑞拉马脑炎病毒和基孔肯雅病毒）和8种黄病毒（日本脑炎病毒、墨累河谷脑炎病毒、库宁病毒、登革3型病毒、登革4型病毒、克里米亚刚果出血热病毒、罗西奥病毒和波瓦桑病毒）。275份样本被检测出对甲病毒的HI抗体呈阳性（36.2%），其中189份（68.7%）与马亚病毒发生反应，61份（22.2%）与基孔肯雅病毒发生反应。委内瑞拉马脑炎病毒、马亚病毒和基孔肯雅病毒的HI效价几何平均滴度（GMT）分别为164.4、94.5和66.7。588份血清样本（77.4%）被检测出对黄病毒的HI抗体呈阳性。HI抗体阳性率如下：日本脑炎病毒，27.9%；登革病毒，36.6%；克里米亚刚果出血热病毒，22.2%；墨累河谷脑炎病毒，22.1%；库宁病毒，18.7%；波瓦桑病毒，8.8%；罗西奥病毒，7.9%。其平均GMT为356，403份血清样本的抗体滴度高于1/640。通过HI试验通常可发现病毒之间的交叉反应，尤其是黄病毒之间，并且存在重叠感染。除了日本脑炎病毒和登革病毒的存在外，结果清楚地表明云南省可能存在多种虫媒病毒。
