Le Lay-Rogues G, Chastel C
Med Trop (Mars). 1984 Jan-Mar;44(1):41-7.
Since 1977, we have performed arboviruses' serosurveys in 330 patients by haemagglutination inhibition test. For 22 of them we have studied specific IgM antibodies against flavivirus. The separation was done by sucrose-gradient density ultracentrifugation and IgM antibodies were demonstrated by the HI test. The 22 IgM tested sera were distributed as follows: 1) 7 positive sera corresponding to patients with a recent flavivirus infection; 2) 12 sera to test with a HI titer greater than or equal to 20 against one or more flaviviruses on a single serum; and 3) 3 negative control sera corresponding to patients with a past known flavivirus infection. For the first group of sera, IgM assay was used to help to determine the causal virus and for the sera to test, it led to conclude to a recent infection in one case and to a past infection in eleven others.
自1977年以来，我们通过血凝抑制试验对330名患者进行了虫媒病毒血清学调查。其中22名患者，我们研究了针对黄病毒的特异性IgM抗体。通过蔗糖梯度密度超速离心法进行分离，并通过血凝抑制试验检测IgM抗体。所检测的22份IgM血清分布如下：1）7份阳性血清对应近期感染黄病毒的患者；2）12份血清单次检测针对一种或多种黄病毒的血凝抑制效价大于或等于20；3）3份阴性对照血清对应既往已知感染黄病毒的患者。对于第一组血清，IgM检测用于辅助确定致病病毒，对于待检测血清，该检测导致一例患者诊断为近期感染，另11例患者诊断为既往感染。
