Oreshkin A E, Miasishcheva N V
Eksp Onkol. 1989;11(2):45-8.
Serum protein transcobalamin II (TC-II) is responsible for transport of cobalamins into mammalian cells. A method of quantitative estimation of plasma membrane receptors of hemopoietic cells to TC-II cobalamin complex is suggested. Analysis of mouse leukemia L1210 cells includes the saturation of radiolabelled ligand-receptor complex with papain. The number of receptors and 57CoCNCbl content in one cell is determined by differentiated radioactivity count of solubilized protein complexes and of cytoplasm.
血清蛋白转钴胺素II（TC-II）负责将钴胺素转运到哺乳动物细胞中。本文提出了一种定量估算造血细胞对TC-II钴胺素复合物的质膜受体的方法。对小鼠白血病L1210细胞的分析包括用木瓜蛋白酶使放射性标记的配体-受体复合物饱和。通过对溶解的蛋白质复合物和细胞质进行差异放射性计数来确定一个细胞中受体的数量和57CoCNCbl的含量。
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