Oreshkin A E, Gudkova M V, Miasishcheva N V
Biull Eksp Biol Med. 1992 Jul;114(8):185-7.
The influence of mitogens on the expression of surface membrane TC-II receptors of human blood lymphocytes and internalization of (TS-II+57Co-CNCbl) complex into cytoplasm were investigated. Mature lymphocytes have a very small number of surface receptors to plasma TC-II but their expression is increased significantly by PHA or Con-A stimulation. CBl transport to cytoplasma is activated in definite sequence by two different mechanisms. Stimulated cells take free CBl without participation of TC-II in early hours of mitogen action (12-42 hrs) before maximal 3H-thymidine incorporation into DNA. On day 3 of cultivation, specific mechanism of CBl transport triggers and the number of lymphoblast receptors is increased manifold. Radioactive CBl enters cytoplasma due to interaction of TC-II-CN [57Co] CBl of the medium with surface membrane receptor of the cells. Thus, the definition of TC-II receptors as an important functional parameter may serve a marker of proliferating cells.
研究了丝裂原对人血淋巴细胞表面膜TC-II受体表达以及（TS-II + 57Co - 钴胺素）复合物内化进入细胞质的影响。成熟淋巴细胞对血浆TC-II的表面受体数量极少，但经PHA或Con - A刺激后其表达显著增加。钴胺素向细胞质的转运通过两种不同机制按一定顺序被激活。在丝裂原作用的早期（12 - 42小时），在最大量的3H - 胸腺嘧啶核苷掺入DNA之前，受刺激的细胞摄取游离钴胺素，此过程不依赖TC-II。在培养的第3天，钴胺素转运的特定机制被触发，淋巴母细胞受体数量增加数倍。放射性钴胺素由于培养基中的TC-II - CN [57Co]钴胺素与细胞表面膜受体相互作用而进入细胞质。因此，将TC-II受体定义为一个重要的功能参数可作为增殖细胞的标志物。
