Zhou Hanying, Zao Wanqiu, Zhang Wei, Shi Juanzi, Shi Wenhao
Assisted Reproduction Center, Maternal & Child Health Care Hospital of Shaanxi Province, Xi'an Houzai Gate 73#, Xi'an 710003, China.
Respiratory Internal Medicine, Shaanxi Provincial People's Hospital, YouYiXilu 236#, Xi'an 710068, China.
Reprod Biomed Online. 2014 Dec;29(6):692-8. doi: 10.1016/j.rbmo.2014.08.011. Epub 2014 Sep 4.
The aim of this study was to evaluate the safety of laser-assisted hatching (LAH) by comparing obstetric and neonatal outcomes between assisted hatching and control groups in cryopreserved embryo transfer cycles. A retrospective cohort analysis was carried out. A total of 699 women with 392 infants delivered were included. Laser- assisted hatching was carried out on D-3 thawed and warmed embryos before transfer in 480 cryopreserved embryos transfer cycles. Obstetric outcomes, neonatal outcomes, and congenital birth defects were recorded. A total of 815 cryopreserved embryo transfer cycles (480 in LAH group and 335 in control group) in 699 patients were analysed. Statistically significantly higher implantation (31.85% versus 16.95%), clinical pregnancy (53.96% versus 33.43%) and live delivery (44.58% versus 23.88%) rates were observed in the LAH group (all P < 0.001). For either singleton or multiple gestations, no statistically significant differences were found in mean gestational age, mean birth weight and mean Apgar score. Four major malformations occurred in the assisted hatching group and three malformations (one major and two minor) in the control group. This study did not identify any harmful effect of LAH on neonates, which suggested that LAH may be a safe treatment in cryopreserved embryo transfer cycles.
本研究的目的是通过比较冷冻胚胎移植周期中辅助孵化组和对照组的产科及新生儿结局，评估激光辅助孵化（LAH）的安全性。进行了一项回顾性队列分析。共纳入699名分娩392名婴儿的女性。在480个冷冻胚胎移植周期中，于移植前对解冻复温后的D-3胚胎进行激光辅助孵化。记录产科结局、新生儿结局和先天性出生缺陷。分析了699例患者的815个冷冻胚胎移植周期（LAH组480个，对照组335个）。LAH组的着床率（31.85%对16.95%）、临床妊娠率（53.96%对33.43%）和活产率（44.58%对23.88%）在统计学上显著更高（均P<0.001）。对于单胎或多胎妊娠，平均孕周、平均出生体重和平均阿氏评分均未发现统计学上的显著差异。辅助孵化组发生4例严重畸形，对照组发生3例畸形（1例严重畸形和2例轻微畸形）。本研究未发现LAH对新生儿有任何有害影响，这表明LAH在冷冻胚胎移植周期中可能是一种安全的治疗方法。
