Kumari Prabla, Poddar Abhijit, Schumann Peter, Das Subrata K
Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar 751 023, Odisha, India.
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Res Microbiol. 2014 Dec;165(10):826-35. doi: 10.1016/j.resmic.2014.10.012. Epub 2014 Nov 4.
A novel marine bacterium, strain LBS2(T) was isolated from eggs carried on pleopods of the spiny lobster collected from Andaman Sea. Heterotrophic growth occurred at 1-7% NaCl. 16S rRNA gene sequence similarity revealed the strain LBS2(T) belonged to the genus Vibrio and showed above 97% similarity with eight type strains of the genus Vibrio. Multilocus analysis based on ftsZ, gapA, gyrB, mreB, pyrH recA, rpoA, and topA revealed LBS2(T) formed a separate cluster with Vibrio ponticus DSM 16217(T) with 89.8% multilocus gene sequence similarity. However, strain LBS2(T) is distantly related with other members of the Scophthalmi clade in terms of 16S rRNA signatures, phenotypic variations and multilocus gene sequence similarity, for which we propose LBS2(T) belongs to a new clade i.e. Ponticus clade with V. ponticus DSM 16217(T) as the representative type strain of the clade. DNA-DNA homologies between strain LBS2(T) and closely related strains were well below 70%. DNA G + C content was 45.3 mol%. On the basis of our polyphasic study, strain LBS2(T) represents a novel species of the genus Vibrio, for which the name Vibrio panuliri sp. nov. is proposed. The type strain is LBS2(T) (= JCM 19500(T) = DSM 27724(T) = LMG 27902(T)).
从安达曼海采集的多刺龙虾腹肢上携带的卵中分离出一种新型海洋细菌，菌株LBS2(T)。该菌在1-7% NaCl条件下进行异养生长。16S rRNA基因序列相似性分析表明，菌株LBS2(T)属于弧菌属，与弧菌属的8个模式菌株相似度高于97%。基于ftsZ、gapA、gyrB、mreB、pyrH、recA、rpoA和topA的多位点分析显示，LBS2(T)与Ponticus弧菌DSM 16217(T)形成一个单独的聚类，多位点基因序列相似度为89.8%。然而，从16S rRNA特征、表型变异和多位点基因序列相似性来看，菌株LBS2(T)与Scophthalmi进化枝的其他成员关系较远，因此我们提议LBS2(T)属于一个新的进化枝，即Ponticus进化枝，以Ponticus弧菌DSM 16217(T)作为该进化枝的代表模式菌株。菌株LBS2(T)与近缘菌株之间的DNA-DNA同源性远低于70%。DNA G + C含量为45.3 mol%。基于我们的多相研究，菌株LBS2(T)代表弧菌属的一个新物种，为此提议将其命名为Vibrio panuliri sp. nov.。模式菌株为LBS2(T)（= JCM 19500(T) = DSM 27724(T) = LMG 27902(T)）。
