Hajyzadeh Mortaza, Turktas Mine, Khawar Khalid Mahmood, Unver Turgay
Cankiri Karatekin University, Faculty of Science, Department of Biology, Cankiri, Turkey.
Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara, Turkey.
Gene. 2015 Jan 25;555(2):186-93. doi: 10.1016/j.gene.2014.11.002. Epub 2014 Nov 4.
Drought stress limits yield severely in most of the crops. Plants utilize complex gene regulation mechanisms to tolerate water deficiency as well as other abiotic stresses. MicroRNAs (miRNAs) are a class of small non-coding RNAs that are progressively recognized as important regulators of gene expression acting at post-transcriptional level. miR408, conserved in terrestrial plants, targets copper related genes. Although, expression level of miR408 is influenced by various environmental factors including drought stress, the biological action of miR408 is still unclear. To examine the miR408 function upon drought stress in chickpea, transgenic lines overexpressing the miR408 were generated. Induced tolerance was observed in the plants with enhanced miR408 expression upon 17-day water deficiency. Expression levels of miR408 target gene together with seven drought responsive genes were measured using qRT-PCR. Here, the involvement of miR408 in drought stress response has been reported. The overexpression leading plantacyanin transcript repression caused regulation of DREB and other drought responsive genes.
干旱胁迫严重限制了大多数作物的产量。植物利用复杂的基因调控机制来耐受水分亏缺以及其他非生物胁迫。微小RNA（miRNA）是一类小的非编码RNA，逐渐被认为是在转录后水平上起作用的重要基因表达调节因子。miR408在陆生植物中保守，靶向与铜相关的基因。虽然miR408的表达水平受包括干旱胁迫在内的各种环境因素影响，但其生物学作用仍不清楚。为了研究鹰嘴豆中miR408在干旱胁迫下的功能，构建了过表达miR408的转基因株系。在缺水17天后，观察到miR408表达增强的植株具有诱导耐受性。使用qRT-PCR检测miR408靶基因以及7个干旱响应基因的表达水平。在此，报道了miR408参与干旱胁迫响应。过表达导致植物花青素转录本抑制，从而调控DREB和其他干旱响应基因。
