Li Wei, Zhang Xin-Cheng, Zhao Jian, Shi Yan, Zhu Xin-Ping
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, PR China; College of Life Science and Fisheries, Shanghai Ocean University, Shanghai 201306, PR China.
Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, PR China.
Gene. 2015 Jan 25;555(2):169-77. doi: 10.1016/j.gene.2014.10.060. Epub 2014 Nov 4.
Cuora trifasciata has become one of the most critically endangered species in the world. The complete mitochondrial genome of C. trifasciata (Chinese three-striped box turtle) was determined in this study. Its mitochondrial genome is a 16,575-bp-long circular molecule that consists of 37 genes that are typically found in other vertebrates. And the basic characteristics of the C. trifasciata mitochondrial genome were also determined. Moreover, a comparison of C. trifasciata with Cuora cyclornata, Cuora pani and Cuora aurocapitata indicated that the four mitogenomics differed in length, codons, overlaps, 13 protein-coding genes (PCGs), ND3, rRNA genes, control region, and other aspects. Phylogenetic analysis with Bayesian inference and maximum likelihood based on 12 protein-coding genes of the genus Cuora indicated the phylogenetic position of C. trifasciata within Cuora. The phylogenetic analysis also showed that C. trifasciata from Vietnam and China formed separate monophyletic clades with different Cuora species. The results of nucleotide base compositions, protein-coding genes and phylogenetic analysis showed that C. trifasciata from these two countries may represent different Cuora species.
三线闭壳龟已成为世界上最濒危的物种之一。本研究测定了三线闭壳龟（中国三线箱龟）的完整线粒体基因组。其线粒体基因组是一个长度为16575 bp的环状分子，由其他脊椎动物中常见的37个基因组成。同时也确定了三线闭壳龟线粒体基因组的基本特征。此外，对三线闭壳龟与潘氏闭壳龟、金头闭壳龟和黄额闭壳龟的比较表明，这四种线粒体基因组在长度、密码子、重叠区、13个蛋白质编码基因（PCGs）、ND3、rRNA基因、控制区等方面存在差异。基于闭壳龟属12个蛋白质编码基因的贝叶斯推断和最大似然法系统发育分析表明了三线闭壳龟在闭壳龟属中的系统发育位置。系统发育分析还表明，来自越南和中国的三线闭壳龟与不同的闭壳龟物种形成了独立的单系分支。核苷酸碱基组成、蛋白质编码基因和系统发育分析结果表明，来自这两个国家的三线闭壳龟可能代表不同的闭壳龟物种。
