Prakash Pravin, Ghosliya Dolly, Gupta Vikrant
Biotechnology Division, CSIR - Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, India.
Biotechnology Division, CSIR - Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, India.
Gene. 2015 Jan 10;554(2):181-95. doi: 10.1016/j.gene.2014.10.046. Epub 2014 Oct 28.
MicroRNAs are small endogenous non-coding RNAs of ~19-24 nucleotides and perform regulatory roles in many plant processes. To identify miRNAs involved in regulatory networks controlling diverse biological processes including secondary metabolism in Catharanthus roseus, an important medicinal plant, we employed deep sequencing of small RNA from leaf tissue. A total of 88 potential miRNAs comprising of 81 conserved miRNAs belonging to 35 families and seven novel miRNAs were identified. Precursors for 16 conserved and seven novel cro-miRNAs were identified, and their stem-loop hairpin structures were predicted. Selected cro-miRNAs were analyzed by stem-loop qRT-PCR and differential expression patterns were observed in different vegetative tissues of C. roseus. Targets were predicted for conserved and novel cro-miRNAs, which were found to be involved in diverse biological role(s) including secondary metabolism. Our study enriches available resources and information regarding miRNAs and their potential targets for better understanding of miRNA-mediated gene regulation in plants.
微小RNA是一类长度约为19 - 24个核苷酸的内源性非编码小RNA，在许多植物过程中发挥调控作用。为了鉴定参与调控包括重要药用植物长春花次生代谢在内的多种生物学过程的调控网络的微小RNA，我们对叶片组织中的小RNA进行了深度测序。共鉴定出88个潜在的微小RNA，其中包括属于35个家族的81个保守微小RNA和7个新的微小RNA。鉴定出了16个保守的和7个新的长春花微小RNA的前体，并预测了它们的茎环发夹结构。通过茎环定量逆转录-聚合酶链反应对选定的长春花微小RNA进行了分析，并在长春花不同的营养组织中观察到了差异表达模式。对保守的和新的长春花微小RNA的靶标进行了预测，发现它们参与了包括次生代谢在内的多种生物学功能。我们的研究丰富了有关微小RNA及其潜在靶标的现有资源和信息，有助于更好地理解植物中微小RNA介导的基因调控。
