Das Moumita, Hasan Mahmudul, Akter Sharmin, Roy Sawrab, Sharma Binayok, Chowdhury Md Shahidur Rahman, Ahsan Md Irtija, Akhand Rubaiat Nazneen, Uddin Md Bashir, Ahmed Syed Sayeem Uddin
Department of Epidemiology and Public Health, Sylhet Agricultural University, Sylhet, Bangladesh.
Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet, Bangladesh.
Bioinform Biol Insights. 2021 Dec 6;15:11779322211046729. doi: 10.1177/11779322211046729. eCollection 2021.
is a protozoan parasite, the etiologic agent of Neosporosis-a common cause of abortion in cattle worldwide. Herd level prevalence of Neosporosis could be as high as 90%. However, there is no approved treatment and vaccines available for Neosporosis. MicroRNA (miRNA) based prophylaxis and therapeutics could be options for Neosporosis in cattle and other animals. The current study aimed to investigate the genome of to identify and characterize the conserved miRNAs through Expressed Sequence Tags (ESTs) dependent homology search. A total of 1,041 mature miRNAs of reference organisms were employed against 336 non-redundant ESTs available in the genome of . The study predicted one putative miRNA "nca-miR-9388-5p" of 19 nucleotides with MFEI value -1.51 kcal/mol and (A + U) content% 72.94% corresponding with its pre-miRNA. A comprehensive search for specific gene targets was performed and discovered 16 potential genes associated with different protozoal physiological functions. Significantly, the gene "Protein phosphatase" was found responsible for the virulence of . The other genes were accounted for gene expression, vesicular transport, cell signaling, cell proliferation, DNA repair mechanism, and different developmental stages of the protozoon. Therefore, this study finding will provide pivotal information to future aspirants upon Bovine Neosporosis. It will also serve as the baseline information for further studies of the bioinformatics approach to identify other protozoal miRNAs.
是一种原生动物寄生虫，是新孢子虫病的病原体，新孢子虫病是全球牛流产的常见原因。新孢子虫病在畜群中的流行率可能高达90%。然而，目前尚无批准用于新孢子虫病的治疗方法和疫苗。基于微小RNA（miRNA）的预防和治疗方法可能是牛及其他动物新孢子虫病的选择。本研究旨在通过依赖表达序列标签（EST）的同源性搜索来研究该寄生虫的基因组，以鉴定和表征保守的miRNA。总共使用了1041个参考生物体的成熟miRNA，针对该寄生虫基因组中336个非冗余EST进行分析。该研究预测了一个推定的miRNA“nca-miR-9388-5p”，其长度为19个核苷酸，MFEI值为-1.51千卡/摩尔，（A+U）含量为72.94%，与其前体miRNA相对应。对特定基因靶点进行了全面搜索，发现了16个与不同原生动物生理功能相关的潜在基因。值得注意的是，发现“蛋白磷酸酶”基因与该寄生虫的毒力有关。其他基因则参与基因表达、囊泡运输、细胞信号传导、细胞增殖、DNA修复机制以及原生动物的不同发育阶段。因此，本研究结果将为未来研究牛新孢子虫病的人员提供关键信息。它也将作为进一步研究生物信息学方法以鉴定其他原生动物miRNA的基础信息。
