Osipova V P, Kolyada M N, Berberova N T, Milaeva E R, Ponomareva E N, Belaya M M
Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia.
Southern Scientific Center, Russian Academy of Sciences, Rostov-on-Don, Russia.
Cryobiology. 2014 Dec;69(3):467-72. doi: 10.1016/j.cryobiol.2014.10.007. Epub 2014 Oct 18.
A cryoprotective effect of an addition of a new synthetic antioxidant - a representative of phosphorus-containing sterically hindered phenols is presented. The efficiency of the compound was shown to exceed the effect of lipid-soluble antioxidants butylated hydroxytoluene (BHT) and trolox in the conditions of cryopreservation of beluga sperm in the presence of the modified Stein's medium. It was shown that the level of carbonyl oxidation by-products, which can react with thiobarbituric acid (TBARS), in beluga sperm was inversely proportional to the motility time of sperm cells. The fertility of beluga sperm increased 2 times upon the addition of phosphorus-containing phenol to a modified Stein's medium. The prospects of the new antioxidant application to improve cryoresistance of beluga sperm in the conditions of cryopreservation for its efficient protection from the peroxidation processes are discussed.
本文介绍了一种新型合成抗氧化剂（一种含磷位阻酚类代表物）添加后的冷冻保护作用。在改良斯坦因培养基存在的情况下对白鲸精子进行冷冻保存时，该化合物的效率超过了脂溶性抗氧化剂丁基羟基甲苯（BHT）和生育三烯酚。结果表明，白鲸精子中可与硫代巴比妥酸（TBARS）反应的羰基氧化副产物水平与精子细胞的运动时间成反比。在改良斯坦因培养基中添加含磷酚后，白鲸精子的受精能力提高了2倍。本文还讨论了这种新型抗氧化剂在冷冻保存条件下提高白鲸精子抗冻性以有效保护其免受过氧化过程影响的应用前景。
