Izawa T, Komabayashi T, Suda K, Kunisada Y, Shinoda S, Tsuboi M
Laboratory for Exercise and Applied Physiology, Tokyo College of Pharmacy.
J Biochem. 1989 Jan;105(1):110-3. doi: 10.1093/oxfordjournals.jbchem.a122603.
The effect of acute exercise (treadmill running) on rat myocardium beta-adrenergic receptors (beta-AR) was studied. beta-AR was identified in purified sarcolemmal membrane fractions and light vesicle fractions. In control hearts, the number of beta-AR was 21.25 +/- 2.25 and 20.89 +/- 2.89 fmol/mg protein (mean +/- SE) in sarcolemmal membranes and light vesicles, respectively. Immediately after a single bout of dynamic exercise, about 35% of beta-AR was transferred from light vesicles to sarcolemmal membranes (p less than 0.05); concomitantly, isoproterenol-stimulated adenylate cyclase activity also significantly increased in sarcolemmal membranes (p less than 0.05). These results suggest that acute exercise provokes the translocation of beta-AR from a presumably intracellular site (light vesicles) to functional membrane fractions (sarcolemmal membranes) in rat myocardium.
研究了急性运动（跑步机跑步）对大鼠心肌β-肾上腺素能受体（β-AR）的影响。在纯化的肌膜组分和轻囊泡组分中鉴定出β-AR。在对照心脏中，肌膜和轻囊泡中β-AR的数量分别为21.25±2.25和20.89±2.89 fmol/mg蛋白质（平均值±标准误）。单次动态运动后立即发现，约35%的β-AR从轻囊泡转移至肌膜（p<0.05）；同时，异丙肾上腺素刺激的腺苷酸环化酶活性在肌膜中也显著增加（p<0.05）。这些结果表明，急性运动促使大鼠心肌中的β-AR从假定的细胞内位点（轻囊泡）转移至功能性膜组分（肌膜）。
