Maisel A S, Motulsky H J, Insel P A
Science. 1985 Oct 11;230(4722):183-6. doi: 10.1126/science.2994229.
beta-Adrenergic receptors were identified in two fractions of guinea pig myocardium: a purified sarcolemmal fraction and a light vesicle (presumably intracellular) fraction. In the light vesicle fraction, which contained approximately 25 percent of the myocardial receptors under control conditions, the receptors appeared to be segregated from the stimulatory guanine nucleotide binding and catalytic components of adenylate cyclase. During myocardial ischemia, beta-adrenergic receptors were redistributed from the intracellular vesicles to the sarcolemmal fraction, where isoproterenol-stimulated adenylate cyclase activity was increased. These findings should facilitate further studies on cellular and molecular mechanisms that regulate adrenergic receptor traffic in the myocardium and may explain the rapid enhancement in adrenergic receptor expression that occurs with myocardial ischemia.
在豚鼠心肌的两个组分中鉴定出了β-肾上腺素能受体：一个纯化的肌膜组分和一个轻囊泡（可能是细胞内的）组分。在轻囊泡组分中，在对照条件下含有约25%的心肌受体，这些受体似乎与腺苷酸环化酶的刺激性鸟嘌呤核苷酸结合和催化成分分离。在心肌缺血期间，β-肾上腺素能受体从细胞内囊泡重新分布到肌膜组分，在那里异丙肾上腺素刺激的腺苷酸环化酶活性增加。这些发现应有助于进一步研究调节心肌中肾上腺素能受体运输的细胞和分子机制，并可能解释心肌缺血时发生的肾上腺素能受体表达的快速增强。
