Liu Sha, Wang Mei-Xia, Mao Cheng-Jie, Cheng Xiao-Yu, Wang Chen-Tao, Huang Jian, Zhong Zhao-Min, Hu Wei-Dong, Wang Fen, Hu Li-Fang, Wang Han, Liu Chun-Feng
Department of Neurology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou 215004, China; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
Center for Circadian Clock, Soochow University, Suzhou, China; School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou 215123, Jiangsu, China.
Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):353-7. doi: 10.1016/j.bbrc.2014.11.021. Epub 2014 Nov 15.
It has been demonstrated that acid sensing ionic channels (ASICs) are present in the central and peripheral nervous system of mammals, including the retina. However, it remains unclear whether the zebrafish retina also expresses ASICs. In the present study, the expression and distribution of zasic1 were examined in the retina of zebrafish. Both zasic1 mRNA and protein expressions were detected in the adult zebrafish retina. A wide distribution of ASIC1 in zebrafish retina was confirmed using whole mount in situ hybridization and immunohistochemistry study. Acidosis-induced currents in the isolated retinal ganglion cells (RGCs) were also recorded using whole cell patch clamping. Moreover, blockade of ASICs channel significantly reduced the locomotion of larval zebrafish in response to light exposure. In sum, our data demonstrate the presence of ASIC1 and its possible functional relevance in the retina of zebrafish.
已经证明，酸敏感离子通道（ASICs）存在于包括视网膜在内的哺乳动物的中枢和外周神经系统中。然而，斑马鱼视网膜是否也表达ASICs仍不清楚。在本研究中，检测了zasic1在斑马鱼视网膜中的表达和分布。在成年斑马鱼视网膜中检测到了zasic1 mRNA和蛋白表达。使用全胚胎原位杂交和免疫组织化学研究证实了ASIC1在斑马鱼视网膜中的广泛分布。还使用全细胞膜片钳记录了分离的视网膜神经节细胞（RGCs）中酸中毒诱导的电流。此外，ASICs通道的阻断显著降低了幼体斑马鱼对光照的运动反应。总之，我们的数据证明了ASIC1在斑马鱼视网膜中的存在及其可能的功能相关性。
