Departamento de Morfología y Biología Celular, Grupo SINPOS, Universidad de Oviedo, Spain.
Neurosci Lett. 2013 Mar 1;536:35-40. doi: 10.1016/j.neulet.2013.01.006. Epub 2013 Jan 14.
In detecting chemical properties of food, different molecules and ion channels are involved including members of the acid-sensing ion channels (ASICs) family. Consistently ASICs are present in sensory cells of taste buds of mammals. In the present study the presence of ASICs (ASIC1, ASIC2, ASIC3 and ASIC4) was investigated in the taste buds of adult zebrafish (zASICs) using Western blot and immunohistochemistry. zASIC1 and zASIC3 were regularly absent from taste buds, whereas faint zASIC2 and robust zASIC4 immunoreactivities were detected in sensory cells. Moreover, zASIC2 also immunolabelled nerves supplying taste buds. The present results demonstrate for the first time the presence of zASICs in taste buds of teleosts, with different patterns to that occurring in mammals, probably due to the function of taste buds in aquatic environment and feeding. Nevertheless, the role of zASICs in taste remains to be demonstrated.
在检测食物的化学特性时，涉及到不同的分子和离子通道，包括酸感应离子通道（ASICs）家族的成员。哺乳动物的味蕾感觉细胞中始终存在 ASICs。在本研究中，使用 Western blot 和免疫组织化学方法研究了成年斑马鱼（zASICs）味蕾中的 ASICs（ASIC1、ASIC2、ASIC3 和 ASIC4）的存在情况。zASIC1 和 zASIC3 通常不存在于味蕾中，而在感觉细胞中检测到微弱的 zASIC2 和强烈的 zASIC4 免疫反应性。此外，zASIC2 还对供应味蕾的神经进行了免疫标记。本研究结果首次证明了 zASICs 在硬骨鱼类味蕾中的存在，其模式与哺乳动物中的不同，这可能是由于味觉在水生环境和摄食中的功能所致。然而，zASICs 在味觉中的作用仍有待证明。
