Fenn Carlin M, Small Brian C
Center for Fisheries, Aquaculture and Aquatic Sciences, Southern Illinois University, Carbondale, IL 62901, USA; Department of Animal Science, Food, and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA.
Center for Fisheries, Aquaculture and Aquatic Sciences, Southern Illinois University, Carbondale, IL 62901, USA; Center for Ecology, Southern Illinois University, Carbondale, IL 62901, USA; Department of Animal Science, Food, and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA.
Comp Biochem Physiol A Mol Integr Physiol. 2015 Feb;180:18-22. doi: 10.1016/j.cbpa.2014.11.002. Epub 2014 Nov 8.
Sturgeon are a unique fish for physiological research as they are long-lived, slow-growing, and late-maturing. Furthermore, sturgeon growth hormones appear to share greater structural and molecular similarity with mammalian somatotropins than teleostean somatotropins. In this study, changes in insulin-like growth factor (IGF)-I and IGF-II mRNA expression and corresponding whole-body growth and composition following 6 weeks of bi-weekly recombinant bovine growth hormone (rbGH) administration in shovelnose sturgeon Scaphirhynchus platorhynchus were evaluated. Fish were injected intraperitoneally with 240 μg rbGH/g body weight or a sesame oil sham. Hepatic IGF-I and IGF-II mRNA abundance was significantly higher (P≤0.02) in rbGH-treated fish, as were length (P<0.001) and weight gain (P<0.001). In addition, proximate whole-body analysis demonstrated a significant (P<0.05) increase in protein composition of rbGH-treated fish versus sham-treated fish. There were no significant differences in whole-body moisture, lipid, or ash between the two treatments. These results demonstrate functional roles for GH and IGFs in the promotion of lean growth within this ancient fish species and support the view that the functional effects of GH on hepatic IGF-I expression and somatic growth are conserved from chondostrean to teleostean fishes.
鲟鱼是生理研究中的一种独特鱼类，因为它们寿命长、生长缓慢且成熟较晚。此外，与硬骨鱼类的生长激素相比，鲟鱼的生长激素在结构和分子上似乎与哺乳动物的生长激素有更大的相似性。在本研究中，评估了铲吻鲟（Scaphirhynchus platorhynchus）每两周注射一次重组牛生长激素（rbGH）6周后，胰岛素样生长因子（IGF）-I和IGF-II mRNA表达的变化以及相应的全身生长和组成情况。给鱼腹腔注射240μg rbGH/g体重或芝麻油作为对照。经rbGH处理的鱼肝脏中IGF-I和IGF-II mRNA丰度显著更高（P≤0.02），体长（P<0.001）和体重增加（P<0.001）也是如此。此外，近似全身分析表明，与假处理的鱼相比，经rbGH处理的鱼蛋白质组成显著增加（P<0.05）。两种处理之间全身水分、脂质或灰分没有显著差异。这些结果证明了生长激素（GH）和胰岛素样生长因子（IGFs）在促进这种古老鱼类瘦生长中的功能作用，并支持了GH对肝脏IGF-I表达和体细胞生长的功能影响从软骨鱼类到硬骨鱼类都是保守的这一观点。
