Gao Yonggang, Zhang Kai, Zhu Fengli, Wu Zhonglin, Chu Xi, Zhang Xuan, Zhang Ying, Zhang Jianping, Chu Li
Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, Hebei, China.
Department of Basic Theory of Traditional Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China.
J Ethnopharmacol. 2014 Dec 2;158 Pt A:397-403. doi: 10.1016/j.jep.2014.10.049. Epub 2014 Oct 31.
Salvia miltiorrhiza (SM, Danshen), a traditional Chinese herbal drug, has been widely used for hundreds of years to treat coronary artery disease.
We studied the effects of SM on the L-type Ca(2+) current (ICa-L) with whole-cell patch-clamp technique in rat ventricular myocytes, and its influence on Ca(2+) transient and contractility using video-based edge detection and dual excitation fluorescence photomultiplier systems as well.
Exposure to SM solution caused a concentration- and voltage-dependent blockade of ICa-L, and the dose of SM solution (10g/l) decreased the maximal inhibitory effect of 35.2±1.2%. However, SM solution did not significantly change the current-voltage relationship or reversal potential of ICa-L, nor did it altered the activation and inactivation gating properties of cardiac Ca(2+) channels. Meanwhile, SM decreased the amplitude of myocyte shortening and the peak value of Ca(2+) transient with a significant decrease in the time to 90% of the baseline (Tr), but the time to 10% of the peak (Tp) was not dramatically prolonged.
The results indicated that SM significantly inhibited L-type Ca(2+) channels, decreased [Ca(2+)]i and contractility in adult rat cardiac myocytes. These findings may be relevant to the cardioprotective efficacy of SM.
丹参，一种传统的中草药，数百年来一直被广泛用于治疗冠状动脉疾病。
我们采用全细胞膜片钳技术研究了丹参对大鼠心室肌细胞L型钙电流（ICa-L）的影响，并使用基于视频的边缘检测和双激发荧光光电倍增管系统研究了其对钙瞬变和收缩性的影响。
暴露于丹参溶液导致ICa-L呈浓度和电压依赖性阻断，丹参溶液剂量（10g/l）使最大抑制作用降低35.2±1.2%。然而，丹参溶液并未显著改变ICa-L的电流-电压关系或反转电位，也未改变心脏钙通道的激活和失活门控特性。同时，丹参降低了心肌细胞缩短幅度和钙瞬变峰值，使达到基线90%的时间（Tr）显著缩短，但达到峰值10%的时间（Tp）并未显著延长。
结果表明，丹参显著抑制成年大鼠心肌细胞的L型钙通道，降低细胞内钙浓度（[Ca(2+)]i）和收缩性。这些发现可能与丹参的心脏保护作用有关。
