Niranjana Murthy Hosakatte, Dandin Vijayalaxmi S, Yoeup Paek Kee
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, Republic of Korea; Department of Botany, Karnatak University, Dharwad 580003, India.
Department of Botany, Karnatak University, Dharwad 580003, India.
J Ethnopharmacol. 2014 Dec 2;158 Pt A:442-6. doi: 10.1016/j.jep.2014.10.047. Epub 2014 Oct 31.
Ginseng (Panax ginseng C. A. Meyer) has a beneficial role in the treatment of various diseases including liver disorders like acute/chronic hepatotoxicity, hepatitis, hepatic fibrosis/cirrhosis and hepatocellular carcinoma.
Tissue culture raised mountain ginseng adventitious root (TCMGARs) extract with ginsenosides in abundance was used as an experimental material. 'Sprague-Dawley' male rats were used as experimental systems and were fed with TCMGARs extracts at doses of 30, 100, 300mg/kg body weight for two weeks to test the effect on carbon tetrachloride (CCl4) induced acute liver damage. Field cultivated Korean ginseng root extract fed rats (100mg/kg) were used as positive control. Plasma enzyme levels, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were assessed. Glutathione (GSH) and malondialdehyde (MDA) concentrations were also evaluated.
TCMGARs extracts remarkably prevented the elevation of ALT, AST, ALP and liver peroxides in CCl4-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extracts in experimental groups.
The TCMGARs rich in varied ginsenosides can afford protection against CCl4-induced hepatocellular injury.
人参（Panax ginseng C. A. Meyer）在治疗各种疾病中具有有益作用，包括肝脏疾病，如急性/慢性肝毒性、肝炎、肝纤维化/肝硬化和肝细胞癌。
以富含人参皂苷的组织培养高山参不定根（TCMGARs）提取物作为实验材料。将“斯普拉格-道利”雄性大鼠作为实验系统，以30、100、300mg/kg体重的剂量喂食TCMGARs提取物两周，以测试其对四氯化碳（CCl4）诱导的急性肝损伤的影响。喂食田间种植的高丽参根提取物的大鼠（100mg/kg）作为阳性对照。评估血浆酶水平，即丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）。还评估了谷胱甘肽（GSH）和丙二醛（MDA）的浓度。
TCMGARs提取物显著预防了CCl4处理大鼠中ALT、AST、ALP和肝脏过氧化物的升高。实验组用提取物处理后，肝脏谷胱甘肽水平显著升高。
富含多种人参皂苷的TCMGARs可对CCl4诱导的肝细胞损伤起到保护作用。
