Andersen K B, Skov H
Department of Biology, Danmarks farmaceutiske Højskole, Københaven, Denmark.
J Gen Virol. 1989 Jul;70 ( Pt 7):1921-7. doi: 10.1099/0022-1317-70-7-1921.
The effect of proteases on retrovirus-induced cell fusion was studied. Both 'fusion from within' (fusion between infected and uninfected cells) and 'fusion from without' (fusion of uninfected cells by virus addition) was enhanced after treatment with proteases. An enhancement of up to 10-fold, giving 30% fusion of the cells, was observed. Protease treatment of infected cells or of virus particles enhanced fusion, indicating that cleavage of the virus surface proteins is important for cell fusion. Cell fusion is believed to reflect the virus-cell membrane fusion which occurs during the entry of enveloped viruses into cells. It is therefore possible that proteolytic cleavage of virus surface components during entry is involved in virus infection.
研究了蛋白酶对逆转录病毒诱导的细胞融合的影响。在用蛋白酶处理后，“内部融合”（感染细胞与未感染细胞之间的融合）和“外部融合”（通过添加病毒使未感染细胞融合）均得到增强。观察到增强倍数高达10倍，使30%的细胞发生融合。对感染细胞或病毒颗粒进行蛋白酶处理可增强融合，这表明病毒表面蛋白的裂解对细胞融合很重要。细胞融合被认为反映了包膜病毒进入细胞过程中发生的病毒-细胞膜融合。因此，有可能在病毒进入过程中病毒表面成分的蛋白水解裂解参与了病毒感染。
