Altanerova V, Portetelle D, Kettmann R, Altaner C
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
J Gen Virol. 1989 Jul;70 ( Pt 7):1929-32. doi: 10.1099/0022-1317-70-7-1929.
Adult rats were infected with bovine leukaemia virus (BLV). Inoculated rats persistently produced antibodies directed against viral structural proteins. No major pathogenesis in infected rats was found during 2 years of observation. It was possible to recover the virus from rat spleen several months after infection. A cell line, R(BLV), was established from rat spleen; this contained integrated BLV provirus. R(BLV) cells kept for over 80 passages in vitro produced viral particles with the properties of BLV. Provirus reintegration and/or amplification occurred in R(BLV) cells. The cell line was found to be tumorigenic in rats, and the virus produced was immunogenic. R(BLV) cells represent the first described BLV-producing rat cell line. Proven persistent infection with BLV indirectly suggests that rats can serve as a reservoir of BLV in nature.
成年大鼠感染了牛白血病病毒（BLV）。接种后的大鼠持续产生针对病毒结构蛋白的抗体。在两年的观察期内，未发现感染大鼠有明显的发病情况。感染数月后，可从大鼠脾脏中分离出病毒。从大鼠脾脏中建立了一个细胞系R(BLV)；该细胞系含有整合的BLV前病毒。R(BLV)细胞在体外传代80多次后产生了具有BLV特性的病毒颗粒。前病毒在R(BLV)细胞中发生了重新整合和/或扩增。发现该细胞系在大鼠中具有致瘤性，所产生的病毒具有免疫原性。R(BLV)细胞系是首个被描述的产生BLV的大鼠细胞系。已证实的BLV持续感染间接表明，大鼠在自然界中可能是BLV的储存宿主。
