Laboratory of Global Infectious Diseases Control Science, Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Viral Infectious Diseases Unit, RIKEN, Saitama, 351-0198, Japan.
Retrovirology. 2022 May 18;19(1):7. doi: 10.1186/s12977-022-00592-6.
The potential risk and association of bovine leukemia virus (BLV) with human remains controversial as it has been reported to be both positive and negative in human breast cancer and blood samples. Therefore, establishing the presence of BLV in comprehensive human clinical samples in different geographical locations is essential.
In this study, we examined the presence of BLV proviral DNA in human blood and breast cancer tissue specimens from Japan. PCR analysis of BLV provirus in 97 Japanese human blood samples and 23 breast cancer tissues showed negative result for all samples tested using long-fragment PCR and highly-sensitive short-fragment PCR amplification. No IgG and IgM antibodies were detected in any of the 97 human serum samples using BLV gp51 and p24 indirect ELISA test. Western blot analysis also showed negative result for IgG and IgM antibodies in all tested human serum samples.
Our results indicate that Japanese human specimens including 97 human blood, 23 breast cancer tissues, and 97 serum samples were negative for BLV.
牛白血病病毒（BLV）与人类之间的潜在风险和关联存在争议，因为有报道称其在人类乳腺癌和血液样本中既有阳性结果，也有阴性结果。因此，在不同地理位置的综合人类临床样本中确定 BLV 的存在至关重要。
在这项研究中，我们检测了来自日本的人类血液和乳腺癌组织标本中 BLV 前病毒 DNA 的存在情况。使用长片段 PCR 和高灵敏度短片段 PCR 扩增对 97 份日本人类血液样本和 23 份乳腺癌组织中的 BLV 前病毒进行 PCR 分析，所有检测样本均呈阴性。使用 BLV gp51 和 p24 间接 ELISA 试验对 97 份人血清样本进行检测，均未检测到 IgG 和 IgM 抗体。Western blot 分析也显示所有检测的人血清样本的 IgG 和 IgM 抗体均呈阴性。
我们的结果表明，包括 97 份人血、23 份乳腺癌组织和 97 份血清样本在内的日本人类标本均为 BLV 阴性。
