Lutz Amanda Brosius
Stanford University School of Medicine, Department of Neurobiology, Stanford, California 94305.
Cold Spring Harb Protoc. 2014 Dec 1;2014(12):1234-6. doi: 10.1101/pdb.top073981.
This article introduces methods for the acute purification and primary culture of Schwann cells from the mouse sciatic nerve. Immunopanning can be used to isolate Schwann cells from intact nerves during early postnatal development as well as to purify Schwann cells from adult nerves following sciatic nerve injury. These methods facilitate the exploration of mouse Schwann cell biology in the healthy and injured peripheral nerve.
本文介绍了从小鼠坐骨神经中急性纯化和原代培养雪旺细胞的方法。免疫淘选可用于在出生后早期从完整神经中分离雪旺细胞，也可用于在坐骨神经损伤后从成年神经中纯化雪旺细胞。这些方法有助于探索健康和损伤的周围神经中小鼠雪旺细胞生物学。
