Mills P J, Dimsdale J E, Ziegler M G
Department of Psychiatry, University of California, San Diego, La Jolla 92093.
Life Sci. 1989;44(25):1917-20. doi: 10.1016/0024-3205(89)90403-7.
Isoproterenol-stimulated cyclic AMP (cAMP) accumulation in lymphocytes is an index of beta-adrenergic receptor functioning. It is not known if a delay between blood collection and subsequent cell preparation influences cAMP accumulation. We compared cAMP levels in lymphocytes processed from whole blood immediately after collection and following a 3.5 hour delay. There were no significant changes in either basal or stimulated levels of cAMP. This information may alleviate time constraints in certain experimental designs where immediate isolation of lymphocytes from whole blood may not be practical.
异丙肾上腺素刺激淋巴细胞中环磷酸腺苷（cAMP）的积累是β-肾上腺素能受体功能的一个指标。目前尚不清楚采血与随后的细胞制备之间的延迟是否会影响cAMP的积累。我们比较了采血后立即从全血中处理的淋巴细胞以及延迟3.5小时后处理的淋巴细胞中的cAMP水平。cAMP的基础水平或刺激水平均无显著变化。这一信息可能会缓解某些实验设计中的时间限制，在这些实验设计中，从全血中立即分离淋巴细胞可能不切实际。
