《Hi-C分析指南：实用准则》
The Hitchhiker's guide to Hi-C analysis: practical guidelines.
作者信息
Lajoie Bryan R, Dekker Job, Kaplan Noam
机构信息
Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605-0103, USA.
出版信息
Methods. 2015 Jan 15;72:65-75. doi: 10.1016/j.ymeth.2014.10.031. Epub 2014 Nov 6.
Abstract
Over the last decade, development and application of a set of molecular genomic approaches based on the chromosome conformation capture method (3C), combined with increasingly powerful imaging approaches, have enabled high resolution and genome-wide analysis of the spatial organization of chromosomes. The aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions.
摘要
在过去十年中，基于染色体构象捕获方法（3C）的一系列分子基因组学方法的开发与应用，再结合日益强大的成像方法，使得对染色体空间组织进行高分辨率全基因组分析成为可能。本文旨在为分析和解释通过全基因组3C方法（如Hi-C和3C-seq）获得的数据提供指导方针，这些方法依赖深度测序来检测和量化成对的染色质相互作用。
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