Grob Stefan, Cavalli Giacomo
Institute of Human Genetics, Centre National de la Recherche UMR9002, Montpellier, France.
Methods Mol Biol. 2018;1675:233-246. doi: 10.1007/978-1-4939-7318-7_14.
The introduction of chromosome conformation capture (3C) technologies boosted the field of 3D-genome research and significantly enhanced the available toolset to study chromosomal architecture. 3C technologies not only offer increased resolution compared to the previously dominant cytological approaches but also allow the simultaneous study of genome-wide 3D chromatin contacts, thereby enabling a candidate-free perspective on 3D-genome architecture. Since its introduction in 2002, 3C technologies evolved rapidly and now constitute a collection of tools, each with their strengths and pitfalls with respect to specific research questions. This chapter aims at guiding 3C novices through the labyrinth of potential applications of the various family members, hopefully providing a valuable basis for choosing the appropriate strategy for different research questions.
染色体构象捕获（3C）技术的引入推动了三维基因组研究领域的发展，并显著扩充了用于研究染色体结构的工具集。与之前占主导地位的细胞学方法相比，3C技术不仅提供了更高的分辨率，还能同时研究全基因组范围内的三维染色质相互作用，从而为三维基因组结构提供了一种无候选基因的研究视角。自2002年问世以来，3C技术发展迅速，如今已形成了一系列工具，每种工具针对特定的研究问题都有其优势和不足。本章旨在引导3C技术的新手走出各种技术潜在应用的迷宫，希望能为针对不同研究问题选择合适的策略提供有价值的依据。
