9-β-D-2'-脱氧呋喃核糖基-1-脱氮嘌呤的立体选择性酶促合成。
The stereoselective enzymatic synthesis of 9-beta-D-2'-deoxyribofuranosyl 1-deazapurine.
作者信息
Betbeder D, Hutchinson D W, Richards A O
机构信息
Department of Chemistry, University of Warwick, Coventry, UK.
出版信息
Nucleic Acids Res. 1989 Jun 12;17(11):4217-22. doi: 10.1093/nar/17.11.4217.
Abstract
The transfer of 2-deoxyribose from thymidine to 1-deazapurine which is catalysed by N-deoxyribosyl transferases from Lactobacillus leichmanii occurs in high yield. This is a very stereoselective process and only one product, 9-beta-D-2'-deoxyribofuranosyl 1-deazapurine, is formed. Nmr spectroscopy, and in particular, nuclear Overhauser enhancement experiments, confirm that the 2-deoxyribose moiety is bound to N-9 rather than N-7 and that the glycosidic link has the beta-configuration.
摘要
由利氏乳酸杆菌的N-脱氧核糖基转移酶催化的2-脱氧核糖从胸苷向1-脱氮嘌呤的转移反应产率很高。这是一个非常立体选择性的过程，只形成一种产物，即9-β-D-2'-脱氧呋喃核糖基1-脱氮嘌呤。核磁共振光谱，特别是核Overhauser增强实验，证实2-脱氧核糖部分与N-9而非N-7相连，且糖苷键具有β构型。
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