Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia 57159-44867, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia 57159-44867, Iran.
Int J Surg. 2014 Dec;12(12):1409-15. doi: 10.1016/j.ijsu.2014.10.023. Epub 2014 Oct 28.
The repair of peripheral nerve injuries is still one of the most challenging tasks and concerns in neurosurgery, plastic and orthopedic surgery. Effect of acetyl-L-carnitine (ALC) loaded chitosan conduit as an in situ delivery system of ALC in bridging the defects was studied using a rat sciatic nerve regeneration model. A 10-mm sciatic nerve defect was bridged using a chitosan conduit (CHIT/ALC) filled with 10 μL ALC (100 ng/mL). In control group (CHIT), the conduit was filled with the same volume of the phosphate buffered solution. The regenerated fibers were studied 4, 8, 12 and 16 weeks after surgery. The functional and electrophysiological studies confirmed faster recovery of the regenerated axons in ALC treated than control group (P < 0.05). The mean ratios of gastrocnemius muscles weight were measured. There was statistically significant difference between the muscle weight ratios of CHIT/ALC and CHIT groups (P<0.05). Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers in CHIT/ALC were significantly higher than in control group. In immuohistochemistry, the location of reactions to S-100 in CHIT/ALC was clearly more positive than CHIT group. ALC when loaded in a chitosan conduit resulted in improvement of functional recovery and quantitative morphometric indices of sciatic nerve.
周围神经损伤的修复仍然是神经外科、整形外科和骨科最具挑战性的任务之一。本研究使用大鼠坐骨神经再生模型，研究了乙酰-L-肉碱（ALC）负载壳聚糖导管作为 ALC 原位递送系统在桥接缺损中的作用。使用填充有 10 μL ALC（100ng/mL）的壳聚糖导管（CHIT/ALC）桥接 10mm 坐骨神经缺损。在对照组（CHIT）中，导管中填充了相同体积的磷酸盐缓冲液。术后 4、8、12 和 16 周研究再生纤维。功能和电生理研究证实，ALC 处理组的再生轴突恢复更快（P<0.05）。测量比目鱼肌重量的平均比值。CHIT/ALC 和 CHIT 组之间的肌肉重量比存在统计学差异（P<0.05）。再生纤维的形态计量学指数表明，CHIT/ALC 中有髓纤维的数量和直径明显高于对照组。在免疫组织化学中，CHIT/ALC 中 S-100 反应的位置明显比 CHIT 组更阳性。当 ALC 装载在壳聚糖导管中时，可改善坐骨神经的功能恢复和定量形态计量学指标。
