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致病性与非致病性溶组织内阿米巴之间的基因组DNA差异。

Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica.

作者信息

Tannich E, Horstmann R D, Knobloch J, Arnold H H

机构信息

Bernhard Nocht Institute for Tropical Medicine, Hamburg, Federal Republic of Germany.

出版信息

Proc Natl Acad Sci U S A. 1989 Jul;86(13):5118-22. doi: 10.1073/pnas.86.13.5118.

DOI:10.1073/pnas.86.13.5118
PMID:2544890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC297568/
Abstract

cDNA libraries were constructed from pathogenic (HM-1:IMSS) and nonpathogenic (SAW 1734) isolates of Entamoeba histolytica. A cDNA clone (cEH-P1) specific for pathogenic amoebae was identified by screening with a pool of sera from patients with invasive amoebiasis that had been absorbed with nonpathogenic amoebae. This clone was used for the identification of a homologous clone (cEH-NP1) in the cDNA from nonpathogenic amoebae. Sequence analysis and comparison of the predicted amino acid sequences for both clones disclosed 12% evolutionary divergence in structure. Hybridization of both cDNA probes to genomic DNA from four pathogenic and five nonpathogenic E. histolytica isolates revealed two distinct Southern blot patterns, one characteristic for pathogenic amoebae and the other for nonpathogenic amoebae. Further, the complex pattern of restriction fragments hybridizing to an actin cDNA probe was also different between pathogenic and nonpathogenic isolates but was conserved within each group of amoebae. The results indicate that pathogenic isolates of E. histolytica are genetically distinct from nonpathogenic isolates.

摘要

从溶组织内阿米巴的致病菌株（HM-1:IMSS）和非致病菌株（SAW 1734）构建了cDNA文库。通过用来自侵袭性阿米巴病患者且已用非致病阿米巴吸收的血清池进行筛选，鉴定出了一种对致病阿米巴特异的cDNA克隆（cEH-P1）。该克隆用于在非致病阿米巴的cDNA中鉴定同源克隆（cEH-NP1）。对两个克隆的预测氨基酸序列进行序列分析和比较，发现结构上有12%的进化差异。两种cDNA探针与来自4个致病和5个非致病溶组织内阿米巴分离株的基因组DNA杂交，显示出两种不同的Southern印迹模式，一种是致病阿米巴的特征模式，另一种是非致病阿米巴的特征模式。此外，与肌动蛋白cDNA探针杂交的限制性片段的复杂模式在致病和非致病分离株之间也不同，但在每组阿米巴内是保守的。结果表明，溶组织内阿米巴的致病分离株在遗传上与非致病分离株不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/478c15fc1691/pnas00280-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/ea94e237a75f/pnas00280-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/01185273f7fc/pnas00280-0325-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/11f6b8a90119/pnas00280-0325-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/478c15fc1691/pnas00280-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/ea94e237a75f/pnas00280-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/01185273f7fc/pnas00280-0325-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/11f6b8a90119/pnas00280-0325-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe3/297568/478c15fc1691/pnas00280-0327-a.jpg

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Effect of culture conditions and bacterial associates on the zymodemes of Entamoeba histolytica.培养条件和细菌共生体对溶组织内阿米巴酶谱型的影响。
Parasitol Today. 1987 Feb;3(2):37-40; discussion 37. doi: 10.1016/0169-4758(87)90210-9.
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New M13 vectors for cloning.用于克隆的新型M13载体。
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and amoebic liver abscess in northern Sri Lanka: a public health problem.斯里兰卡北部的阿米巴肝脓肿：一个公共卫生问题。
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