[HLA-DP和DQ基因单核苷酸多态性与慢性乙型肝炎病毒感染结局的相关性研究]

[Studies on the association of single nucleotide polymorphisms of HLA-DP and DQ genes with the outcome of chronic hepatitis B virus infection].

作者信息

Su Mingkuan, Zeng Yongbin, Chen Jing, Jiang Ling, Chen Tianbin, Liu Can, Yang Bin, Ou Qishui

机构信息

The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian 350005, P. R. China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014 Dec;31(6):765-9. doi: 10.3760/cma.j.issn.1003-9406.2014.06.019.

DOI:10.3760/cma.j.issn.1003-9406.2014.06.019

PMID:25449085

Abstract

OBJECTIVE

To investigate the association of single nucleotide polymorphisms in the HLA-DP and DQ genes with the outcome of chronic hepatitis B virus infection.

METHODS

Two hundred and four healthy subjects, 255 clearance subjects, 204 asymptomatic HBV carriers (AsC), 136 chronic hepatitis B (CHB), 68 liver cirrhosis (LC) and hepatocellular carcinoma (HCC) were enrolled. Genotypes of rs3077, rs9277535 and rs2647050 were determined by sequence specific primers-PCR (PCR-SSP).

RESULTS

By using healthy subjects and clearance subjects as the control groups, rs3077 and rs9277535 were significantly associated with chronic HBV infection under additive and dominant models (P< 0.05). Meanwhile, haplotypes GGA, AGA, AAA appeared to be protective factors against chronic HBV infection (P < 0.05). By using AsC as the control group, comparison with the CHB, LC and HCC groups showed no association of the 3 SNPs or haplotypes with the clinical outcome (P > 0.05).

CONCLUSION

HLA-DP gene polymorphisms are strongly associated with chronic HBV infection. The presence of A allele at rs3077 and rs9277535 of the HLA-DP gene may decreased the risk for chronic HBV infection.

摘要

目的

研究人类白细胞抗原-DP（HLA-DP）和-DQ基因单核苷酸多态性与慢性乙型肝炎病毒感染结局的相关性。

方法

纳入204名健康受试者、255名病毒清除受试者、204名无症状HBV携带者（AsC）、136名慢性乙型肝炎（CHB）患者、68名肝硬化（LC）患者和肝细胞癌（HCC）患者。采用序列特异性引物聚合酶链反应（PCR-SSP）检测rs3077、rs9277535和rs2647050的基因型。

结果

以健康受试者和病毒清除受试者作为对照组，在加性和显性模型下，rs3077和rs9277535与慢性HBV感染显著相关（P<0.05）。同时，单倍型GGA、AGA、AAA似乎是慢性HBV感染的保护因素（P<0.05）。以AsC作为对照组，与CHB、LC和HCC组比较，3个单核苷酸多态性或单倍型与临床结局均无相关性（P>0.05）。