HLA-DP基因rs2281388多态性与肝细胞癌风险之间的相关性。
Relevance between HLA-DP gene rs2281388 polymorphism and hepatocellular carcinoma risk.
作者信息
Liu Fangfeng, Wang Jianlu, Chang Hong, Lu Jun, Li Hongguang
机构信息
Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 9677 Jingshi Road, Jinan 250021, Shandong, China.
Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiiated to Shandong University 9677 Jingshi Road, Jinan 250021, Shandong, China.
出版信息
Int J Clin Exp Pathol. 2015 Jun 1;8(6):7431-5. eCollection 2015.
PURPOSE
We carried out this study to find out the relevance between rs2281388 T/C polymorphism of human leukocyte antigen (HLA) gene and hepatocellular carcinoma (HCC) risk in Chinese Han population.
METHODS
The method of polymerase chain reaction (PCR) was applied to amplify the genomic DNA. Then the PCR products were sequenced to test the HLA-DP gene rs2281388T/C polymorphism of the case and control groups. Odds ratios (ORs) and 95% confidence interval (95% CIs) were utilized to evaluate the potential correlation between rs2281388 variants and HCC risk.
RESULTS
We analyzed the rs2281388 polymorphism distribution among the clinical pathological features. The results showed that there existed a significant statistic correlation between rs2281388T/C polymorphism of HLA-DP gene and HBsAg feature, and no significant correlation was found between rs2281388 and other clinical features. Further analysis showed that the TT genotype of rs2281388 was significantly correlated with HCC risk, and the same to T allele, but there was no significant difference of CT genotype distribution in case and control groups.
CONCLUSION
TT genotype and T allele of HLA-DP gene rs2281388 polymorphism may increase the risk of HCC.
目的
我们开展本研究以探究人类白细胞抗原（HLA）基因rs2281388 T/C多态性与中国汉族人群肝细胞癌（HCC）风险之间的相关性。
方法
应用聚合酶链反应（PCR）方法扩增基因组DNA。然后对PCR产物进行测序，以检测病例组和对照组的HLA - DP基因rs2281388T/C多态性。采用优势比（OR）和95%置信区间（95%CI）来评估rs2281388变异与HCC风险之间的潜在相关性。
结果
我们分析了rs2281388多态性在临床病理特征中的分布情况。结果显示，HLA - DP基因的rs2281388T/C多态性与HBsAg特征之间存在显著的统计学相关性，而rs2281388与其他临床特征之间未发现显著相关性。进一步分析表明，rs2281388的TT基因型与HCC风险显著相关，T等位基因也是如此，但病例组和对照组中CT基因型分布无显著差异。
结论
HLA - DP基因rs2281388多态性的TT基因型和T等位基因可能增加HCC风险。
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