携带大片段插入片段的源自pBR322的多拷贝质粒在大肠杆菌K-12中通常是二聚体。
pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia coli K-12.
作者信息
Berg C M, Liu L, Coon M, Strausbaugh L D, Gray P, Vartak N B, Brown M, Talbot D, Berg D E
机构信息
Molecular and Cell Biology Department, University of Connecticut, Storrs 06269-2131.
出版信息
Plasmid. 1989 Mar;21(2):138-41. doi: 10.1016/0147-619x(89)90057-7.
PMID:2544913
Abstract
pBR322-related plasmids that are 2.3 to 5.1 kb were found predominantly as monomers, while plasmids that are 7.7 to 15.2 kb were found predominantly as dimers in rec+ cells of Escherichia coli K-12.
摘要
在大肠杆菌K-12的rec+细胞中，发现2.3至5.1千碱基对的pBR322相关质粒主要以单体形式存在，而7.7至15.2千碱基对的质粒主要以二聚体形式存在。
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