Keenan Molly R, Leung Sarah J, Rice Photini S, Wall R Andrew, Barton Jennifer K
The University of Arizona, Department of Biomedical Engineering, 1657 E. Helen Street, 210240, Tucson, Arizona, 85721.
Lasers Surg Med. 2015 Jan;47(1):30-9. doi: 10.1002/lsm.22307. Epub 2014 Dec 1.
We utilize a miniature, dual-modality endoscope that combines fluorescence-based surface magnifying chromoendoscopy (SMC) and optical coherence tomography (OCT) to follow the anatomical changes that occur during adenoma development in the mouse colon.
Twenty-five mice were treated with the carcinogen azoxymethane (AOM) to induce tumor development in the distal colon, or were treated with saline as control, and were imaged over six months. OCT detects adenoma number with high sensitivity and specificity and can measure lesion size. In methylene blue-lavaged colons, SMC detects changes in the colonic crypts. SMC images of control mouse colons exhibit reticulated patterns of crypts of equal size, forming either a dot or honeycomb pattern.
Images of AOM-treated colons show mild crypt irregularities even in grossly normal tissue. Images of small to medium adenoma exhibit larger crypts, more intense signal, and irregular spacing whereas those of large adenoma have heterogeneous, intense signal and loss of crypt structure.
The combination of OCT and SMC permits the detection of neoplastic events from the earliest stages of crypt irregularities before gross tissue changes are noted, through to measuring the growth of protruding adenoma.
我们使用一种微型双模态内窥镜，它结合了基于荧光的表面放大染色内镜检查（SMC）和光学相干断层扫描（OCT），以追踪小鼠结肠腺瘤发生过程中出现的解剖学变化。
25只小鼠接受致癌物偶氮甲烷（AOM）处理以诱导远端结肠肿瘤发生，或接受生理盐水处理作为对照，并在六个月内进行成像。OCT能以高灵敏度和特异性检测腺瘤数量，并可测量病变大小。在亚甲蓝灌洗的结肠中，SMC可检测结肠隐窝的变化。对照小鼠结肠的SMC图像显示大小相等的隐窝呈网状图案，形成点状或蜂窝状图案。
AOM处理的结肠图像显示，即使在大体正常的组织中也有轻度隐窝不规则。中小腺瘤的图像显示隐窝更大、信号更强且间距不规则，而大腺瘤的图像则信号不均匀、强烈且隐窝结构消失。
OCT和SMC的联合使用能够检测从隐窝最早出现不规则（在注意到大体组织变化之前）到测量突出腺瘤生长等肿瘤发生事件。
