Greening Gage J, James Haley M, Powless Amy J, Hutcheson Joshua A, Dierks Mary K, Rajaram Narasimhan, Muldoon Timothy J
Department of Biomedical Engineering, University of Arkansas, 1 University of Arkansas, Fayetteville, AR 72701, USA.
Department of Chemistry and Biochemistry, University of Arkansas, 1 University of Arkansas, Fayetteville, AR 72701, USA.
Biomed Opt Express. 2015 Nov 19;6(12):4934-50. doi: 10.1364/BOE.6.004934. eCollection 2015 Dec 1.
Early detection of structural or functional changes in dysplastic epithelia may be crucial for improving long-term patient care. Recent work has explored myriad non-invasive or minimally invasive "optical biopsy" techniques for diagnosing early dysplasia, such as high-resolution microendoscopy, a method to resolve sub-cellular features of apical epithelia, as well as broadband sub-diffuse reflectance spectroscopy, a method that evaluates bulk health of a small volume of tissue. We present a multimodal fiber-based microendoscopy technique that combines high-resolution microendoscopy, broadband (450-750 nm) sub-diffuse reflectance spectroscopy (sDRS) at two discrete source-detector separations (374 and 730 μm), and sub-diffuse reflectance intensity mapping (sDRIM) using a 635 nm laser. Spatial resolution, magnification, field-of-view, and sampling frequency were determined. Additionally, the ability of the sDRS modality to extract optical properties over a range of depths is reported. Following this, proof-of-concept experiments were performed on tissue-simulating phantoms made with poly(dimethysiloxane) as a substrate material with cultured MDA-MB-468 cells. Then, all modalities were demonstrated on a human melanocytic nevus from a healthy volunteer and on resected colonic tissue from a murine model. Qualitative in vivo image data is correlated with reduced scattering and absorption coefficients.
早期检测发育异常上皮细胞的结构或功能变化对于改善患者的长期护理可能至关重要。最近的研究探索了多种用于诊断早期发育异常的非侵入性或微创“光学活检”技术，例如高分辨率显微内镜检查（一种用于分辨顶端上皮细胞亚细胞特征的方法）以及宽带亚漫反射光谱法（一种评估小体积组织整体健康状况的方法）。我们提出了一种基于光纤的多模态显微内镜技术，该技术结合了高分辨率显微内镜检查、在两个离散源探测器间距（374和730μm）下的宽带（450 - 750nm）亚漫反射光谱法（sDRS）以及使用635nm激光的亚漫反射强度映射（sDRIM）。确定了空间分辨率、放大倍数、视野和采样频率。此外，还报告了sDRS模式在一系列深度上提取光学特性的能力。在此之后，对以聚二甲基硅氧烷为基底材料并培养了MDA - MB - 468细胞的组织模拟体模进行了概念验证实验。然后，在一名健康志愿者的人类黑素细胞痣以及来自小鼠模型的切除结肠组织上展示了所有模式。体内定性图像数据与降低的散射和吸收系数相关。
