野生型及抗锑的巴西利什曼原虫和婴儿利什曼原虫品系中铁超氧化物歧化酶-A的功能分析
Functional analysis of iron superoxide dismutase-A in wild-type and antimony-resistant Leishmania braziliensis and Leishmania infantum lines.
作者信息
Tessarollo N G, Andrade J M, Moreira D S, Murta S M F
机构信息
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou CPqRR/Fiocruz, Belo Horizonte, MG, Brazil.
Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou CPqRR/Fiocruz, Belo Horizonte, MG, Brazil.
出版信息
Parasitol Int. 2015 Apr;64(2):125-9. doi: 10.1016/j.parint.2014.11.001. Epub 2014 Nov 7.
In this work, we characterized the gene encoding iron superoxide dismutase-A (FeSOD-A) in wild-type (WTS) and antimony-resistant (SbR) L. (Viannia) braziliensis and L. (Leishmania) infantum lines, which were selected in vitro. FeSOD-A transcript and protein expression were similar in all tested lines; however, specific enzyme activity analysis revealed higher superoxide dismutase activity in SbIII-resistant LbSbR and LiSbR lines than in the corresponding WTS lines. These parasites were also more tolerant to oxidative stress generated by the herbicide paraquat. Functional analysis showed that in comparison to non-transfected lines, wild-type LbWTS and LiWTS clones overexpressing the FeSOD-A enzyme are 1.6- and 1.7-fold more resistant to SbIII, respectively. Our results suggest that FeSOD-A is involved in the antimony resistance phenotype in L. (V.) braziliensis and L. (L.) infantum.
在这项研究中，我们对野生型（WTS）和抗锑（SbR）的巴西利什曼原虫（L. (Viannia) braziliensis）及婴儿利什曼原虫（L. (Leishmania) infantum）品系中编码铁超氧化物歧化酶-A（FeSOD-A）的基因进行了表征，这些品系是在体外筛选得到的。在所有测试品系中，FeSOD-A转录本和蛋白表达相似；然而，特定酶活性分析显示，抗SbIII的LbSbR和LiSbR品系中的超氧化物歧化酶活性高于相应的WTS品系。这些寄生虫对除草剂百草枯产生的氧化应激也更具耐受性。功能分析表明，与未转染品系相比，过表达FeSOD-A酶的野生型LbWTS和LiWTS克隆对SbIII的抗性分别提高了1.6倍和1.7倍。我们的结果表明，FeSOD-A参与了巴西利什曼原虫和婴儿利什曼原虫的抗锑表型。
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