Fragoso José Manuel, Zuñiga-Ramos Joaquín, Arellano-González Marva, Alvarez-León Edith, Villegas-Torres Beatriz E, Cruz-Lagunas Alfredo, Delgadillo-Rodriguez Hilda, Peña-Duque Marco Antonio, Martínez-Ríos Marco Antonio, Vargas-Alarcón Gilberto
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico; Interventional Genetic Study Group in Cardiovascular Disease's, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Department of Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City, Mexico.
Exp Mol Pathol. 2015 Feb;98(1):13-7. doi: 10.1016/j.yexmp.2014.11.007. Epub 2014 Nov 13.
The aim of the present study was to establish the role of IL-6 and TGF-β1 gene polymorphisms in the risk of developing in-stent restenosis. Two IL-6 [rs1800796 (-572 G>C), rs2069827 (-1426 T>G)] and two TGF-β1 [rs1800469 (-509 T>C), rs1800470 (T29C)] gene polymorphisms were analyzed by 5' exonuclease TaqMan genotyping assays in a group of 244 patients, who underwent coronary artery stenting. Basal and procedure coronary angiography were analyzed, looking for angiographic predictors of restenosis and follow-up angiography was performed to screen for binary restenosis. Under the dominant and additive models adjusted for hypertension, stable angina, stent used, and diameter of stent, the TGF-β1 T29C (rs1800470) polymorphism was significantly associated with an increase risk of restenosis when compared to patients without restenosis (OR=2.06, 95% CI: 1.03-4.11, P(Dom)=0.034 and OR=1.64, 95% CI: 1.09-2.45, PAdd=0.016). TGF-β1 polymorphisms were in linkage disequilibrium and one haplotype (TT) was significantly increased in patients with restenosis when compared to patients without restenosis (OR=2.03, P=0.041). In summary, our results suggest that the TGF-β1 T29C gene polymorphism could be involved in the risk of developing restenosis after coronary stent placement.
本研究的目的是确定白细胞介素-6（IL-6）和转化生长因子-β1（TGF-β1）基因多态性在支架内再狭窄发生风险中的作用。通过5'核酸外切酶TaqMan基因分型检测法，对一组244例行冠状动脉支架置入术的患者分析了两个IL-6基因多态性位点[rs1800796（-572 G>C）、rs2069827（-1426 T>G）]和两个TGF-β1基因多态性位点[rs1800469（-509 T>C）、rs1800470（T29C）]。分析了基础冠状动脉造影和手术冠状动脉造影，寻找再狭窄的血管造影预测指标，并进行随访血管造影以筛查二元再狭窄。在针对高血压、稳定型心绞痛、使用的支架和支架直径进行校正的显性和加性模型下，与无再狭窄的患者相比，TGF-β1 T29C（rs1800470）多态性与再狭窄风险增加显著相关（优势比[OR]=2.06，95%可信区间[CI]：1.03 - 4.11，显性模型P=0.034；OR=1.64，95%CI：1.09 - 2.45，加性模型P=0.016）。TGF-β1多态性处于连锁不平衡状态，与无再狭窄的患者相比，再狭窄患者中的一种单倍型（TT）显著增加（OR=2.03，P=0.041）。总之，我们的结果表明，TGF-β1 T29C基因多态性可能与冠状动脉支架置入术后再狭窄的发生风险有关。
