Tsujimoto Hiroyuki, Tanzawa Ayumi, Miyamoto Hiroe, Horii Tsunehito, Tsuji Misaki, Kawasumi Akari, Tamura Atsushi, Wang Zhen, Abe Rie, Tanaka Shota, Yamanaka Kouki, Matoba Mari, Torii Hiroko, Ozamoto Yuki, Takamori Hideki, Suzuki Shuko, Morita Shinichiro, Ikada Yoshito, Hagiwara Akeo
Division of Medical Life System, Department of Life and Medical Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
Research and Development Department, Gunze Ltd., Ayabe, Kyoto, 623-8511, Japan.
J Biomed Mater Res B Appl Biomater. 2015 Oct;103(7):1511-8. doi: 10.1002/jbm.b.33340. Epub 2014 Nov 29.
In order to prevent postoperative adhesion and the related complications, a thermally crosslinked gelatin (TCG) film was developed and the basic biological properties were examined, paying special attention to the relationship between these properties and the extent of crosslinking of the film. The gelatin films crosslinked thermally for five different time periods (0, 1, 3, 8, and 14 hours) were developed and the following tests were performed. Regarding the material characterization of the films, the water content, the water solubility, and the enzymatic degradation for collagenase were found to be closely related to the duration of thermal crosslinking. In an in vitro study conducted to examine the cell growth of fibroblasts cultured on the films, the degree of cell growth, except no crosslinked film, was less than that observed in the control group, thus suggesting that such effects of the films on fibroblast cell growth may be related with their anti-adhesive effects. In in vivo tests, the films crosslinked for longer time periods (3, 8, and 14 hours) were retained for longer after being implanted into the abdominal cavity in rats and showed a significant anti-adhesive effect in the rat cecum adhesion models, indicating that the biodegradability and anti-adhesive effects of the TCG films depend on the duration of thermal crosslinking. In order to develop useful and effective anti-adhesive gelatin film, it is very important to optimize duration of the thermal crosslinking.
为了预防术后粘连及相关并发症，研发了一种热交联明胶（TCG）薄膜并对其基本生物学特性进行了检测，特别关注这些特性与薄膜交联程度之间的关系。制备了在五个不同时间段（0、1、3、8和14小时）进行热交联的明胶薄膜，并进行了以下测试。关于薄膜的材料特性，发现水分含量、水溶性和胶原酶的酶解作用与热交联持续时间密切相关。在一项体外研究中，检测在薄膜上培养的成纤维细胞的生长情况，除未交联的薄膜外，细胞生长程度均低于对照组，这表明此类薄膜对成纤维细胞生长的影响可能与其抗粘连作用有关。在体内试验中，交联时间较长（3、8和14小时）的薄膜植入大鼠腹腔后保留时间更长，并且在大鼠盲肠粘连模型中显示出显著的抗粘连效果，这表明TCG薄膜的生物降解性和抗粘连作用取决于热交联的持续时间。为了研发有用且有效的抗粘连明胶薄膜，优化热交联持续时间非常重要。
