Regueiro Leticia, Carballa Marta, Lema Juan M
J Biotechnol. 2014 Dec 20;192 Pt A:179-86. doi: 10.1016/j.jbiotec.2014.10.007.
To improve the stability of anaerobic reactors, more knowledge is required about how the different communities react against operating perturbations and which specific ones respond better. The objective of this work was to monitor the changes in microbial community structure of an anaerobic digester during a temperature drop by applying different complementary molecular techniques. Temperature decrease led to an increase of Bacteroidales order, Porphyromonadaceae family and Bacteroides genus and a decrease in Syntrophomonas and Clostridium genera. Once the temperature was restored, the reactor recovered the steady state performance without requiring any modification in operational conditions or in the microbiome. During the recovery period, Sedimentibacter genus and Porphyromonadaceae family played an important role in the degradation of the accumulated volatile fatty acids. The hydrogenotrophic methanogens appeared to be the keystone archaeal population at low temperatures as well as in the recovery period. This study stands out that the understanding of microbial community dynamics during temperature drop could be utilized to develop strategies for the mitigation of temperature change consequences and speed up the recovery of stable reactor performance.
为提高厌氧反应器的稳定性，需要更多关于不同群落如何应对运行扰动以及哪些特定群落反应更好的知识。本研究的目的是通过应用不同的互补分子技术，监测厌氧消化器在温度下降期间微生物群落结构的变化。温度降低导致拟杆菌目、卟啉单胞菌科和拟杆菌属增加，而互营单胞菌属和梭菌属减少。温度恢复后，反应器无需对运行条件或微生物群落进行任何调整即可恢复稳态性能。在恢复期，沉积杆菌属和卟啉单胞菌科在积累的挥发性脂肪酸降解中发挥了重要作用。氢营养型产甲烷菌似乎是低温期和恢复期的关键古菌种群。本研究突出表明，了解温度下降期间的微生物群落动态可用于制定减轻温度变化影响的策略，并加快稳定反应器性能的恢复。
