Lee Noo Ri, Kim Hyun-Soo, Kim You-Sun, Kwon Myung-Hee, Choi Kyeong Sook, Lee Chang-Woo
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Biochem Biophys Res Commun. 2014 Nov 21;454(3):429-35. doi: 10.1016/j.bbrc.2014.10.103. Epub 2014 Oct 28.
A minor fraction of cohesin complexes at chromosome arms is not removed by the prophase pathway, and maintained until metaphase and enriched at centromeres. Sgo1 localizes to chromosome arms from prophase to metaphase, and is indispensable for removing cohesin complexes from chromosome arms. However, it has not been established how the chromosome arm localization of Sgo1 leads to the establishment of cohesion on chromosomes. Here, we report that Aurora B kinase interacts with and phosphorylates Sgo1 in vitro and in vivo. Furthermore, the phosphorylation of Sgol by Aurora B kinase regulated the distribution of Sgo1 between centromeres and chromosome arms, and the expression of Aurora B kinase-dead mutants of Sgo1 caused mislocalization from centromeres to chromosome arms. These results suggest Aurora B kinase directly regulates the subcellular distribution of Sgo1 to facilitate the accurate separation of mitotic chromosomes.
染色体臂上一小部分黏连蛋白复合体不会通过前期途径被去除，而是维持到中期并在着丝粒处富集。Sgo1从前期到中期定位于染色体臂，对于从染色体臂上去除黏连蛋白复合体是不可或缺的。然而，Sgo1在染色体臂上的定位如何导致染色体上黏连的建立尚未明确。在此，我们报告Aurora B激酶在体外和体内与Sgo1相互作用并使其磷酸化。此外，Aurora B激酶对Sgol的磷酸化调节了Sgo1在着丝粒和染色体臂之间的分布，并且Sgo1的Aurora B激酶失活突变体的表达导致其从着丝粒到染色体臂的错误定位。这些结果表明，Aurora B激酶直接调节Sgo1的亚细胞分布，以促进有丝分裂染色体的准确分离。
